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Selene Aguilera, Ariel Alvarez-Morales, Jesús Murillo, José Luis Hernández-Flores, Jaime Bravo, Susana De la Torre-Zavala
Temperature-mediated biosynthesis of the phytotoxin phaseolotoxin by Pseudomonas syringae pv. phaseolicola depends on the autoregulated expression of the phtABC genes
www.plosone.org
www.plosone.org
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(ligases,)Tj
3.0047 0 Td
(respectively,)Tj
5.1079 0 Td
(are)Tj
1.4513 0 Td
(undoubtedly)Tj
5.3178 0 Td
(involved)Tj
3.6453 0 Td
(in)Tj
1.0375 0 Td
(phaseolotoxin)Tj
5.828 0 Td
(synthesis)Tj
3.8267 0 Td
([)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(15)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.3946 0 Td
(20)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
(].)Tj
-34.0834 -1.3039 Td
(Additionally,)Tj
5.4538 0 Td
(a)Tj
0.652 0 Td
(putative)Tj
3.4411 0 Td
(nonribosomal)Tj
5.845 0 Td
(peptide)Tj
3.1975 0 Td
(synthetase)Tj
4.3483 0 Td
(\(gene)Tj
2.4207 0 Td
(PSPPH_4550\),)Tj
6.1058 0 Td
(located)Tj
3.0784 0 Td
(outside)Tj
-34.5426 -1.2982 Td
(the)Tj
1.4683 0 Td
(Pht)Tj
1.6044 0 Td
(cluster,)Tj
3.0954 0 Td
(is)Tj
0.8504 0 Td
(also)Tj
1.7687 0 Td
(necessary)Tj
4.0195 0 Td
(for)Tj
1.389 0 Td
(phaseolotoxin)Tj
5.828 0 Td
(production)Tj
4.7168 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(].)Tj
-23.5443 -1.2983 Td
(Phaseolotoxin)Tj
5.8733 0 Td
(is)Tj
0.8504 0 Td
(produced)Tj
4.0421 0 Td
(at)Tj
0.9524 0 Td
(relatively)Tj
3.8438 0 Td
(low)Tj
1.6554 0 Td
(temperatures,)Tj
5.7146 0 Td
(allowing)Tj
3.617 0 Td
(studies)Tj
2.9877 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(thermo-)Tj
-33.4768 -1.3039 Td
(regulation)Tj
4.3086 0 Td
(of)Tj
1.0148 0 Td
(genes)Tj
2.4434 0 Td
(involved)Tj
3.6453 0 Td
(in)Tj
1.0375 0 Td
(phaseolotoxin)Tj
5.8279 0 Td
(biosynthesis)Tj
5.1024 0 Td
([)Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
(].)Tj
2.0806 0 Td
(The)Tj
1.7801 0 Td
(working)Tj
3.5603 0 Td
(model)Tj
2.744 0 Td
(for)Tj
1.3889 0 Td
(the)Tj
-34.9338 -1.2983 Td
(induction)Tj
4.1329 0 Td
(of)Tj
1.0148 0 Td
(genes)Tj
2.4434 0 Td
(involved)Tj
3.6509 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(synthesis)Tj
3.8211 0 Td
(of)Tj
1.0148 0 Td
(phaseolotoxin)Tj
5.828 0 Td
(in)Tj
/F8 1 Tf
1.0375 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9921 0 Td
[()7(\n)-13()-9()12()13(\r)2( )6()]TJ
/F7 1 Tf
3.4299 0 Td
(pv.)Tj
1.4173 0 Td
(phaseolicola)Tj
-31.2885 -1.2983 Td
(NPS3121)Tj
3.8947 0 Td
(proposes)Tj
3.7814 0 Td
(that)Tj
1.7801 0 Td
(the)Tj
1.4683 0 Td
(temperature)Tj
5.1307 0 Td
(downshifts)Tj
4.5864 0 Td
(are)Tj
1.4514 0 Td
(sensed)Tj
2.8629 0 Td
(through)Tj
3.4469 0 Td
(an)Tj
1.2019 0 Td
(unknown)Tj
4.0819 0 Td
(mecha-)Tj
-33.6866 -1.2982 Td
(nism,)Tj
2.4377 0 Td
(and)Tj
1.7348 0 Td
(then)Tj
2.0183 0 Td
(the)Tj
1.4683 0 Td
(signal)Tj
2.5398 0 Td
(is)Tj
0.8503 0 Td
(processed)Tj
4.1329 0 Td
(and)Tj
1.7348 0 Td
(transduced)Tj
4.6602 0 Td
(to)Tj
1.0261 0 Td
(an)Tj
1.2019 0 Td
(effector)Tj
3.2428 0 Td
(molecule)Tj
3.8381 0 Td
(that)Tj
1.7801 0 Td
(will)Tj
1.6611 0 Td
(act)Tj
-34.3272 -1.3039 Td
(upon)Tj
2.3187 0 Td
(the)Tj
1.4683 0 Td
(repressor)Tj
3.9288 0 Td
(of)Tj
1.0147 0 Td
(phaseolotoxin)Tj
5.828 0 Td
(biosynthesis)Tj
5.108 0 Td
(genes)Tj
2.4378 0 Td
([)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(].)Tj
2.0806 0 Td
(At)Tj
1.2019 0 Td
(28C,)Tj
2.4491 0 Td
(genes)Tj
2.4435 0 Td
(involved)Tj
3.651 0 Td
(in)Tj
1.0318 0 Td
(pha-)Tj
-34.9622 -1.2983 Td
(seolotoxin)Tj
4.3483 0 Td
(synthesis)Tj
3.8211 0 Td
(are)Tj
1.4512 0 Td
(negatively)Tj
4.2349 0 Td
(regulated)Tj
3.9402 0 Td
(by)Tj
1.1792 0 Td
(a)Tj
0.6576 0 Td
(repressor)Tj
3.9231 0 Td
(molecule)Tj
3.8381 0 Td
(that)Tj
1.7802 0 Td
(might)Tj
2.5965 0 Td
(bind)Tj
2.0693 0 Td
(to)Tj
1.0261 0 Td
(DNA)Tj
-34.8658 -1.2983 Td
(motifs)Tj
2.7609 0 Td
(that)Tj
1.7745 0 Td
(have)Tj
2.0636 0 Td
(previously)Tj
4.3482 0 Td
(been)Tj
2.126 0 Td
(postulated)Tj
4.3596 0 Td
(to)Tj
1.0262 0 Td
(be)Tj
1.1508 0 Td
(involved)Tj
3.6454 0 Td
(in)Tj
1.0374 0 Td
(thermoregulation)Tj
7.2567 0 Td
([)Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7348 0 Td
(27)Tj
0 g
(].)Tj
1.7404 0 Td
(When)Tj
-35.0245 -1.3039 Td
(temperature)Tj
5.125 0 Td
(is)Tj
0.8504 0 Td
(downshifted)Tj
5.1816 0 Td
(to)Tj
1.0261 0 Td
(18C,)Tj
2.4491 0 Td
(the)Tj
1.4684 0 Td
(signal)Tj
2.5454 0 Td
(is)Tj
0.8448 0 Td
(processed)Tj
4.1385 0 Td
(and)Tj
1.7291 0 Td
(relieves)Tj
3.1918 0 Td
(repression)Tj
4.371 0 Td
(mediated)Tj
-32.9212 -1.2982 Td
(by)Tj
1.1792 0 Td
(the)Tj
1.4683 0 Td
(repressor;)Tj
4.1556 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.6519 0 Td
(result,)Tj
2.6758 0 Td
(genes)Tj
2.4435 0 Td
(required)Tj
3.634 0 Td
(for)Tj
1.3889 0 Td
(phaseolotoxin)Tj
5.828 0 Td
(synthesis)Tj
3.8268 0 Td
(are)Tj
1.4456 0 Td
(actively)Tj
3.2202 0 Td
(expressed)Tj
-32.9326 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(].)Tj
1.1962 -1.3039 Td
(The)Tj
1.7801 0 Td
(regulatory)Tj
4.3143 0 Td
(circuitry)Tj
3.6226 0 Td
(controlling)Tj
4.6431 0 Td
(expression)Tj
4.4617 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4627 0 Td
(Pht)Tj
1.6044 0 Td
(cluster)Tj
2.8686 0 Td
(is)Tj
0.8391 0 Td
(apparently)Tj
4.4617 0 Td
(very)Tj
1.9218 0 Td
(complex)Tj
-34.1911 -1.2983 Td
(and)Tj
1.7291 0 Td
(depends)Tj
3.5489 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4684 0 Td
(activity)Tj
3.118 0 Td
(of)Tj
1.0147 0 Td
(several)Tj
2.9367 0 Td
(regulatory)Tj
4.32 0 Td
(genes,)Tj
2.6645 0 Td
(differentially)Tj
5.3065 0 Td
(affecting)Tj
3.6566 0 Td
(its)Tj
1.1452 0 Td
(five)Tj
1.6498 0 Td
(tran-)Tj
-33.834 -1.2983 Td
(scriptional)Tj
4.4617 0 Td
(units.)Tj
2.4491 0 Td
(Phaseolotoxin)Tj
5.8676 0 Td
(production)Tj
4.7168 0 Td
(in)Tj
/F8 1 Tf
1.0375 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9921 0 Td
[()7(\n)-13()-9()12()13(\r)2( )6()]TJ
/F7 1 Tf
3.4299 0 Td
(pv.)Tj
1.4173 0 Td
(phaseolicola)Tj
5.1137 0 Td
(NPS3121)Tj
3.8947 0 Td
(requires)Tj
-33.3804 -1.3039 Td
(the)Tj
1.4683 0 Td
(activity)Tj
3.1181 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(global)Tj
2.6248 0 Td
(regulators)Tj
4.2293 0 Td
(GacS/GacA)Tj
4.8528 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(])Tj
1.8539 0 Td
(and)Tj
1.7291 0 Td
(IHF)Tj
1.8539 0 Td
(\(Integration)Tj
5.0626 0 Td
(Host)Tj
2.1543 0 Td
(Factor\),)Tj
3.3449 0 Td
(which)Tj
-34.7751 -1.2982 Td
(binds)Tj
2.4321 0 Td
(to)Tj
1.0204 0 Td
(the)Tj
1.474 0 Td
(promoter)Tj
4.0308 0 Td
(region)Tj
2.8063 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
/F8 1 Tf
1.4683 0 Td
[()10()22()]TJ
/F7 1 Tf
2.194 0 Td
(operon)Tj
3.0954 0 Td
(exerting)Tj
3.4866 0 Td
(a)Tj
0.652 0 Td
(negative)Tj
3.5319 0 Td
(effect)Tj
2.3698 0 Td
(on)Tj
1.2699 0 Td
(the)Tj
1.474 0 Td
(expression)Tj
4.4617 0 Td
(of)Tj
-36.782 -1.2983 Td
(this)Tj
1.6724 0 Td
(operon)Tj
3.0954 0 Td
([)Tj
0.83 0.64 0.02 0 k
(28)Tj
0 g
(].)Tj
2.075 0 Td
(In)Tj
1.1111 0 Td
(addition,)Tj
3.821 0 Td
(the)Tj
1.4683 0 Td
(expression)Tj
4.4674 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
/F8 1 Tf
1.4684 0 Td
[()10()22()]TJ
/F7 1 Tf
2.3754 0 Td
(operon)Tj
3.0954 0 Td
(depends)Tj
3.5489 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4684 0 Td
(activity)Tj
3.1181 0 Td
(of)Tj
-35.0756 -1.2983 Td
(gene)Tj
/F8 1 Tf
2.0806 0 Td
[()10()22()]TJ
/F7 1 Tf
(,)Tj
2.2507 0 Td
(also)Tj
1.7688 0 Td
(included)Tj
3.7132 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4684 0 Td
(Pht)Tj
1.6044 0 Td
(cluster)Tj
2.8743 0 Td
([)Tj
0.83 0.64 0.02 0 k
(18)Tj
0 g
(].)Tj
2.0749 0 Td
(Furthermore,)Tj
5.6126 0 Td
(genes)Tj
/F8 1 Tf
2.4434 0 Td
[()10()22()-7()]TJ
/F7 1 Tf
(,)Tj
3.5717 0 Td
(located)Tj
3.0784 0 Td
(into)Tj
1.8425 0 Td
(the)Tj
-35.4214 -1.3039 Td
(Pht)Tj
1.6044 0 Td
(cluster,)Tj
3.0954 0 Td
(participate)Tj
4.4729 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(transcriptional)Tj
6.1115 0 Td
(repression)Tj
4.371 0 Td
(of)Tj
1.0148 0 Td
(gene)Tj
/F8 1 Tf
2.0806 0 Td
[( )16()6(\r)]TJ
/F7 1 Tf
2.126 0 Td
(at)Tj
0.9524 0 Td
(temperatures)Tj
5.4878 0 Td
(not)Tj
1.5761 0 Td
(per-)Tj
-35.3987 -1.2983 Td
(missive)Tj
3.1747 0 Td
(for)Tj
1.3833 0 Td
(phaseolotoxin)Tj
5.8279 0 Td
(biosynthesis)Tj
5.108 0 Td
(\(28C\))Tj
2.9027 0 Td
([)Tj
0.83 0.64 0.02 0 k
(29)Tj
0 g
(].)Tj
2.0806 0 Td
(This)Tj
1.9843 0 Td
(repression)Tj
4.371 0 Td
(is)Tj
0.8503 0 Td
(mediated)Tj
3.9402 0 Td
(by)Tj
1.1848 0 Td
(a)Tj
0.652 0 Td
(protein)Tj
-33.4598 -1.2982 Td
(present)Tj
3.1634 0 Td
(in)Tj
1.0318 0 Td
(both)Tj
2.0636 0 Td
(toxigenic)Tj
3.8834 0 Td
(and)Tj
1.7291 0 Td
(nontoxigenic)Tj
5.4935 0 Td
(strains)Tj
2.8573 0 Td
(of)Tj
/F8 1 Tf
1.0148 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F8 1 Tf
0.9978 0 Td
[()7(\n)-13()-9()12()13(\r)2( )6()]TJ
/F7 1 Tf
3.4299 0 Td
(and)Tj
1.7291 0 Td
(requires)Tj
3.4753 0 Td
(the)Tj
1.4683 0 Td
(coordinated)Tj
-32.3373 -1.3039 Td
(participation)Tj
5.3688 0 Td
(of)Tj
1.0147 0 Td
(the)Tj
/F8 1 Tf
1.4684 0 Td
[()10()22()-7()]TJ
/F7 1 Tf
3.3447 0 Td
(products)Tj
3.7531 0 Td
(in)Tj
1.0375 0 Td
(order)Tj
2.4207 0 Td
(to)Tj
1.0262 0 Td
(carry)Tj
2.2733 0 Td
(out)Tj
1.5534 0 Td
(an)Tj
1.2019 0 Td
(efficient)Tj
3.4469 0 Td
(repression)Tj
4.3767 0 Td
(of)Tj
1.0148 0 Td
(gene)Tj
/F8 1 Tf
2.0806 0 Td
[( )16()6(\r)]TJ
/F7 1 Tf
-35.3817 -1.2983 Td
([)Tj
0.83 0.64 0.02 0 k
(29)Tj
0 g
(].)Tj
1.1962 -1.2983 Td
(In)Tj
1.1112 0 Td
(this)Tj
1.6724 0 Td
(study,)Tj
2.6192 0 Td
(we)Tj
1.3266 0 Td
(investigate)Tj
4.4616 0 Td
(the)Tj
1.4683 0 Td
(participation)Tj
5.3688 0 Td
(of)Tj
/F8 1 Tf
1.0148 0 Td
[()10()22()]TJ
/F7 1 Tf
1.5024 0 Td
(genes)Tj
2.4434 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(regulation)Tj
4.3087 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(operons)Tj
-33.4825 -1.3039 Td
(coded)Tj
2.6305 0 Td
(into)Tj
1.8368 0 Td
(the)Tj
1.474 0 Td
(Pht)Tj
1.6044 0 Td
(cluster.)Tj
3.0954 0 Td
(To)Tj
1.3379 0 Td
(this)Tj
1.6724 0 Td
(end,)Tj
1.9446 0 Td
(we)Tj
1.3323 0 Td
(conducted)Tj
4.4277 0 Td
(transcriptional)Tj
6.1114 0 Td
(analyses)Tj
3.4809 0 Td
(of)Tj
/F8 1 Tf
1.0148 0 Td
[()10()22()]TJ
/F7 1 Tf
1.5024 0 Td
(genes)Tj
2.4434 0 Td
(in)Tj
-35.9089 -1.2982 Td
(several)Tj
2.931 0 Td
(mutants)Tj
3.5092 0 Td
(unable)Tj
2.9083 0 Td
(to)Tj
1.0261 0 Td
(produce)Tj
3.5149 0 Td
(phaseolotoxin.)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 347.4708 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Phaseo)Tj
3.3307 0 Td
(lotoxin)Tj
3.0543 0 Td
(biosynthes)Tj
4.6914 0 Td
(is)Tj
0.9284 0 Td
(depends)Tj
3.9898 0 Td
(on)Tj
1.311 0 Td
(phtAB)Tj
2.6788 0 Td
(C)Tj
0.9354 0 Td
(genes)Tj
2.8914 0 Td
(expression)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01784)Tj
4.089 0 Td
(41)Tj
0 g
2.0906 0 Td
(June)Tj
2.3528 0 Td
(1,)Tj
1.0417 0 Td
(2017)Tj
35.5113 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
0.496 0 Td
(15)Tj
ET
Q
q
0.83 0.64 0.02 0 k
1 j
1 J
0 w
36 707.6409 m
42.7465 707.6409 l
h
f*
7.9999 0 0 7.9999 36 708.7181 cm
BT
/F11 1 Tf
1 TL
-0.025 Tc
0 0 Td
(es)Tj
0 g
(\),)Tj
1.5945 0 Td
(co-financ)Tj
3.437 0 Td
(ed)Tj
1.1197 0 Td
(by)Tj
1.1126 0 Td
(the)Tj
1.3677 0 Td
(Fondo)Tj
2.537 0 Td
(Europeo)Tj
3.3166 0 Td
(de)Tj
-14.4851 -1.3748 Td
(Desarrollo)Tj
4.0181 0 Td
(Regional)Tj
3.4583 0 Td
(\(FEDER\))Tj
3.3803 0 Td
(to)Tj
0.9496 0 Td
(JM.)Tj
1.6158 0 Td
(The)Tj
1.5874 0 Td
(funders)Tj
-15.0095 -1.3748 Td
(had)Tj
1.5874 0 Td
(no)Tj
1.1693 0 Td
(role)Tj
1.6157 0 Td
(in)Tj
0.8859 0 Td
(study)Tj
2.2606 0 Td
(design,)Tj
2.9055 0 Td
(data)Tj
1.793 0 Td
(collecti)Tj
2.5653 0 Td
(on)Tj
1.1693 0 Td
(and)Tj
-15.952 -1.3748 Td
(analysis,)Tj
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