Now showing items 1-5 of 5

    • Analysis of stemness gene expression and CD133 abnormal methylation in neuroblastoma cell lines 

      Schiapparelli, Paula; Enguita Germán, Mónica; Balbuena, Jana; Rey, Juan A.; Lázcoz, Paula Upna; Castresana, Javier S. (Spandidos Publications, 2010)   Artículo / Artikulua  OpenAccess
      Neuroblastoma is the most common extracranial solid tumor in children, accounting for up to 10% of all childhood malignancies. Cellular heterogeneity is a hallmark of this embryonal cancer, as distinct neural crest lineages ...
    • Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment 

      Celay Leoz, Ion; Blanco Luquin, Idoia; Lázcoz, Paula Upna; Rotinen Díaz, Mirja Sofia; Castresana, Javier S.; Encío Martínez, Ignacio Upna (Public Library of Science, 2013)   Artículo / Artikulua  OpenAccess
      To determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9-cis RA or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers ...
    • Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR 

      Lorente, Aiala; Mueller, Wolf; Urdangarín, Edurne; Lázcoz, Paula Upna; Deimling, Andreas von; Sáez Castresana, Javier (BioMed Central, 2008)   Artículo / Artikulua  OpenAccess
      Background: We present two melting curve analysis (MCA)-based semiquantitative real time PCR techniques to detect the promoter methylation status of genes. The first, MCA-MSP, follows the same principle as standard MSP ...
    • Frequent promoter hypermethylation of RASSF1A and CASP8 in neuroblastoma 

      Lázcoz, Paula Upna; Muñoz, Jorge; Nistal, Manuel; Pestaña, Ángel; Encío Martínez, Ignacio Upna; Sáez Castresana, Javier (BioMed Central, 2006)   Artículo / Artikulua  OpenAccess
      Background: Epigenetic alterations and loss of heterozygosity are mechanisms of tumor suppressor gene inactivation. A new carcinogenic pathway, targeting the RAS effectors has recently been documented. RASSF1A, on 3p21.3, ...
    • Promoter methylation of RASSF1A associates to adult secondary glioblastomas and pediatric glioblastomas 

      Muñoz, Jorge; Inda, María del Mar; Lázcoz, Paula Upna; Zazpe, Idoya; Fan, Xing; Alfaro, Jorge; Tuñón Álvarez, Teresa; Rey, Juan A.; Castresana, Javier S. (Hindawi / Wiley, 2012)   Artículo / Artikulua  OpenAccess
      While allelic losses and mutations of tumor suppressor genes implicated in the etiology of astrocytoma have been widely assessed, the role of epigenetics is still a matter of study. We analyzed the frequency of promoter ...