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dc.creatorCrespo, Helenaes_ES
dc.creatorReina Arias, Ramséses_ES
dc.creatorGlaría Ezquer, Idoiaes_ES
dc.creatorRamírez, Hugoes_ES
dc.creatorAndrés, Ximena dees_ES
dc.creatorJauregui, Paulaes_ES
dc.creatorLuján, Lluíses_ES
dc.creatorMartínez Pomares, Luisaes_ES
dc.creatorAmorena Zabalza, Beatrizes_ES
dc.creatorAndrés Cara, Damián dees_ES
dc.date.accessioned2015-09-30T07:35:23Z
dc.date.available2015-09-30T07:35:23Z
dc.date.issued2011
dc.identifier.issn0928-4249 (print)
dc.identifier.issn1297-9716 (electronic)
dc.identifier.urihttps://hdl.handle.net/2454/18321
dc.description.abstractThis study aims to characterize the mannose receptor (MR) gene in sheep and its role in ovine visna/maedi virus (VMV) infection. The deduced amino acid sequence of ovine MR was compatible with a transmembrane protein having a cysteine-rich ricin-type amino-terminal region, a fibronectin type II repeat, eight tandem C-type lectin carbohydrate-recognition domains (CRD), a transmembrane region, and a cytoplasmic carboxy-terminal tail. The ovine and bovine MR sequences were closer to each other compared to human or swine MR. Concanavalin A (ConA) inhibited VMV productive infection, which was restored by mannan totally in ovine skin fibroblasts (OSF) and partially in blood monocyte-derived macrophages (BMDM), suggesting the involvement of mannosylated residues of the VMV ENV protein in the process. ConA impaired also syncytium formation in OSF transfected with an ENV-encoding pN3-plasmid. MR transcripts were found in two common SRLV targets, BMDM and synovial membrane (GSM) cells, but not in OSF. Viral infection of BMDM and especially GSM cells was inhibited by mannan, strongly suggesting that in these cells the MR is an important route of infection involving VMV Env mannosylated residues. Thus, at least three patterns of viral entry into SRLV-target cells can be proposed, involving mainly MR in GSM cells (target in SRLV-induced arthritis), MR in addition to an alternative route in BMDM (target in SRLV infections), and an alternative route excluding MR in OSF (target in cell culture). Different routes of SRLV infection may thus coexist related to the involvement of MR differential expression.en
dc.description.sponsorshipThis work was supported by project CICYT Nos. AGL2007-66874-C04-01/GAN and AGL2010-22341-C04-01. We acknowledge the Public University of Navarra and CSIC for fellowships and contract (HC and RR).en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherBioMed Centralen
dc.relation.ispartofVeterinary Research 2011, 42:28en
dc.rights© 2011 Crespo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/
dc.subjectMaedi Visnaen
dc.subjectNucleotide-sequenceen
dc.subjectDendritic cellen
dc.subjectMacrophagesen
dc.subjectExpressionen
dc.subjectBinding proteinsen
dc.subjectCarbohydrateen
dc.subjectRecognitionen
dc.subjectBrainen
dc.titleIdentification of the ovine mannose receptor and its possible role in Visna/Maedi virus infectionen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1186/1297-9716-42-28
dc.relation.publisherversionhttps://dx.doi.org/10.1186/1297-9716-42-28
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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© 2011 Crespo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
La licencia del ítem se describe como © 2011 Crespo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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