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dc.creatorLópez López, Nahikaries_ES
dc.date.accessioned2016-01-13T16:41:01Z
dc.date.available2020-06-01T23:00:11Z
dc.date.issued2015
dc.identifier.urihttps://hdl.handle.net/2454/19853
dc.description.abstractDrought stress is the major factor in the reduction of crop yields causing significant economic losses. However, this stress is difficult to standardize under controlled conditions for research purposes. For molecular biology studies, which usually need highly standardized protocols for screening of large populations, PEG, mannitol or NaCl are employed to apply water deficit conditions. However, these osmolites do not properly simulate drought stress effects. In the present study, a simple experimental approach employing different agar concentration to simulate drought stress conditions in Petri dishes is tested with Medicago truncatula seedlings. The response of root morphology, respiration and drought stress markers is characterized under mild (≈ -1.0 MPa) and moderate drought (≈ -1,5MPa). Mild drought provoked an increase of root length that was not observed under moderate drought. Root area, volume and diameter decrease progressively with the increase of drought severity. Respiration rate was only significantly affected under moderate drought conditions, without changes in cytochrome and alternative pathways capacity. According to this, sucrose, malate and -ketoglutarate content decreased concomitantly with the increase of stress severity. Typical drought stress markers, as proline and amino acids, were also accumulated. Overall, these results validate this simple protocol to study drought stress response of seedlings under controlled conditions.es_ES
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.subjectDroughtes_ES
dc.subjectRootes_ES
dc.subjectMedicago truncatulaes_ES
dc.subjectRespirationes_ES
dc.subjectRoot morphologyes_ES
dc.titleA simple experimental approach to simulate drought stress conditions in agar Petri dishes: characterization of the response in Medicago truncatula plantletses_ES
dc.typeTrabajo Fin de Máster/Master Amaierako Lanaes
dc.typeinfo:eu-repo/semantics/masterThesisen
dc.date.updated2016-01-12T13:30:19Z
dc.contributor.affiliationEscuela Técnica Superior de Ingenieros Agrónomoses_ES
dc.contributor.affiliationNekazaritza Ingeniarien Goi Mailako Eskola Teknikoaeu
dc.description.degreeMáster Universitario en Agrobiología Ambientales_ES
dc.description.degreeUnibertsitate Masterra Ingurumen Agrobiologianeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.embargo.terms2020-06-01es_ES
dc.contributor.advisorTFEGonzález García, Estheres_ES
dc.contributor.advisorTFESeminario Huárriz, Amaiaes_ES


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