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dc.creatorGil Puig, Carmenes_ES
dc.creatorSolano Goñi, Cristinaes_ES
dc.creatorBurgui Erice, Saioaes_ES
dc.creatorLatasa Osta, Cristinaes_ES
dc.creatorGarcía Martínez, Begoñaes_ES
dc.creatorToledo Arana, Alejandroes_ES
dc.creatorLasa Uzcudun, Íñigoes_ES
dc.creatorValle Turrillas, Jaionees_ES
dc.date.accessioned2016-11-04T15:15:58Z
dc.date.available2016-11-04T15:15:58Z
dc.date.issued2014
dc.identifier.issn0019-9567 (Print)
dc.identifier.issn1098-5522 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/22574
dc.descriptionIncluye dos ficheros de datoses_ES
dc.description.abstractThe Staphylococcus aureus biofilm mode of growth is associated with several chronic infections that are very difficult to treat due to the recalcitrant nature of biofilms to clearance by antimicrobials. Accordingly, there is an increasing interest in preventing the formation of S. aureus biofilms and developing efficient antibiofilm vaccines. Given the fact that during a biofilm-associated infection, the first primary interface between the host and the bacteria is the self-produced extracellular matrix, in this study we analyzed the potential of extracellular proteins found in the biofilm matrix to induce a protective immune response against S. aureus infections. By using proteomic approaches, we characterized the exoproteomes of exopolysaccharide-based and proteinbased biofilm matrices produced by two clinical S. aureus strains. Remarkably, results showed that independently of the nature of the biofilm matrix, a common core of secreted proteins is contained in both types of exoproteomes. Intradermal administration of an exoproteome extract of an exopolysaccharide-dependent biofilm induced a humoral immune response and elicited the production of interleukin 10 (IL-10) and IL-17 in mice. Antibodies against such an extract promoted opsonophagocytosis and killing of S. aureus. Immunization with the biofilm matrix exoproteome significantly reduced the number of bacterial cells inside a biofilm and on the surrounding tissue, using an in vivo model of mesh-associated biofilm infection. Furthermore, immunized mice also showed limited organ colonization by bacteria released from the matrix at the dispersive stage of the biofilm cycle. Altogether, these data illustrate the potential of biofilm matrix exoproteins as a promising candidate multivalent vaccine against S. aureus biofilm-associated infections.en
dc.description.sponsorshipJ. Valle was supported by Spanish Ministry of Science and Innovation “Ramón y Cajal” contract. This research was supported by grants ERANET Pathogenomic (GEN2006-27792-C2-1-E/PAT), BIO2011-30503- C02-02, and AGL2011-23954 from the Spanish Ministry of Economy and Competitivity and IIQ14066.RI1 from Innovation Department of the Government of Navarra.en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofInfection and Immunity, March 2014, vol. 82, no. 3, 1017-1029en
dc.rights© 2014, American Society for Microbiology. All Rights Reserved.en
dc.rights© del material complementario: los autoreses_ES
dc.subjectStaphylococcus aureusen
dc.subjectBiofilmsen
dc.subjectExtracellular proteinsen
dc.subjectImmune responseen
dc.titleBiofilm matrix exoproteins induce a protective immune response against Staphylococcus aureus biofilm infectionen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.departmentIdAB - Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1128/IAI.01419-13
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/BIO2011-30503en
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/AGL2011-23954en
dc.relation.publisherversionhttps://dx.doi.org/10.1128/IAI.01419-13
dc.type.versionVersión aceptada / Onetsi den bertsioaes
dc.type.versioninfo:eu-repo/semantics/acceptedVersionen
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua: IIQ14066.RI1


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