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dc.creatorLatasa Osta, Cristinaes_ES
dc.creatorGarcía Martínez, Begoñaes_ES
dc.creatorEcheverz Sarasúa, Maitees_ES
dc.creatorToledo Arana, Alejandroes_ES
dc.creatorValle Turrillas, Jaionees_ES
dc.creatorCampoy Sánchez, Susanaes_ES
dc.creatorGarcía del Portillo, Franciscoes_ES
dc.creatorSolano Goñi, Cristinaes_ES
dc.creatorLasa Uzcudun, Íñigoes_ES
dc.date.accessioned2016-11-04T15:38:36Z
dc.date.available2016-11-04T15:38:36Z
dc.date.issued2012
dc.identifier.issn0021-9193 (Print)
dc.identifier.issn1098-5530 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/22578
dc.descriptionIncluye 1 fichero de datoses_ES
dc.description.abstractThe Rcs phosphorelay pathway is a complex signaling pathway involved in the regulation of many cell surface structures in enteric bacteria. In response to environmental stimuli, the sensor histidine kinase (RcsC) autophosphorylates and then transfers the phosphate through intermediary steps to the response regulator (RcsB), which, once phosphorylated, regulates gene expression. Here, we show that Salmonella biofilm development depends on the phosphorylation status of RcsB. Thus, unphosphorylated RcsB, hitherto assumed to be inactive, is essential to activate the expression of the biofilm matrix compounds. The prevention of RcsB phosphorylation either by the disruption of the phosphorelay at the RcsC or RcsD level or by the production of a nonphosphorylatable RcsB allele induces biofilm development. On the contrary, the phosphorylation of RcsB by the constitutive activation of the Rcs pathway inhibits biofilm development, an effect that can be counteracted by the introduction of a nonphosphorylatable RcsB allele. The inhibition of biofilm development by phosphorylated RcsB is due to the repression of CsgD expression, through a mechanism dependent on the accumulation of the small noncoding RNA RprA. Our results indicate that unphosphorylated RcsB plays an active role for integrating environmental signals and, more broadly, that RcsB phosphorylation acts as a key switch between planktonic and sessile life-styles in Salmonella enterica serovar Typhimurium.en
dc.description.sponsorshipA.T.-A. and J.V. were supported by Ramón y Cajal contracts from the Ministerio de Ciencia e Innovación, Spain. This research was supported by grants BIO2008-05284-C02-01 and BIO2010-18885 from the Spanish Ministerio de Ciencia e Innovación and IIM13329.RI1 from the Gobierno de Navarra (Spain).en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofJournal of Bacteriology, July 2012, vol. 194, no. 14, 3708-3722en
dc.rights© 2012, American Society for Microbiology. All Rights Reserved.en
dc.rights© del material complementario: los autoreses_ES
dc.subjectSalmonellaen
dc.subjectBiofilmen
dc.subjectRscCDBen
dc.subjectCsgDen
dc.subjectUnphosphorylated response regulatoren
dc.titleSalmonella biofilm development depends on the phosphorylation status of RcsBen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.departmentUniversidad Pública de Navarra. Instituto de Agrobiotecnologíaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Agrobioteknologiako Institutuaeu
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1128/JB.00361-12
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/BIO2008-05284
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/BIO2010-18885
dc.relation.publisherversionhttps://dx.doi.org/10.1128/JB.00361-12
dc.type.versionVersión aceptada / Onetsi den bertsioaes
dc.type.versioninfo:eu-repo/semantics/acceptedVersionen
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua: IIM13329.RI1


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