Human papillomavirus detection from human immunodeficiency virus-infected Colombian women’s paired urine and cervical samples
Fecha
2013Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1371/journal.pone.0056509
Resumen
Infection, coinfection and type-specific human papillomavirus (HPV) distribution was evaluated in human immunodeficiency
virus (HIV)-positive women from paired cervical and urine samples. Paired cervical and urine samples (n = 204) were
taken from HIV-positive women for identifying HPV-DNA presence by using polymerase chain reaction (PCR) with three
generic primer sets (GP5+/6+, MY09/11 and pU ...
[++]
Infection, coinfection and type-specific human papillomavirus (HPV) distribution was evaluated in human immunodeficiency
virus (HIV)-positive women from paired cervical and urine samples. Paired cervical and urine samples (n = 204) were
taken from HIV-positive women for identifying HPV-DNA presence by using polymerase chain reaction (PCR) with three
generic primer sets (GP5+/6+, MY09/11 and pU1M/2R). HPV-positive samples were typed for six high-risk HPV (HR-HPV)
(HPV-16, -18, -31, -33, -45 and -58) and two low-risk (LR-HPV) (HPV-6/11) types. Agreement between paired sample results
and diagnostic performance was evaluated. HPV infection prevalence was 70.6% in cervical and 63.2% in urine samples.
HPV-16 was the most prevalent HPV type in both types of sample (66.7% in cervical samples and 62.0% in urine) followed by
HPV-31(47.2%) in cervical samples and HPV-58 (35.7%) in urine samples. There was 55.4% coinfection (infection by more
than one type of HPV) in cervical samples and 40.2% in urine samples. Abnormal Papanicolau smears were observed in
25.3% of the women, presenting significant association with HPV-DNA being identified in urine samples. There was poor
agreement of cervical and urine sample results in generic and type-specific detection of HPV. Urine samples provided the
best diagnosis when taking cytological findings as reference. In conclusion including urine samples could be a good
strategy for ensuring adherence to screening programs aimed at reducing the impact of cervical cancer, since this sample is
easy to obtain and showed good diagnostic performance. [--]
Materias
Human papillomavirus (HPV),
HIV,
Urine samples,
Cervical samples
Editor
Public Library of Science
Publicado en
PLoS ONE 8(2): e56509
Departamento
Universidad Pública de Navarra. Departamento de Estadística e Investigación Operativa /
Nafarroako Unibertsitate Publikoa. Estatistika eta Ikerketa Operatiboa Saila
Versión del editor
Entidades Financiadoras
This project was funded by the Basque Cooperation Agency Development and by the Spanish Agency for International Development Cooperation
(AECID).