Local inhibition of nitrogen fixation and nodule metabolism in drought-stressed soybean
Gil Quintana, Erena
Larrainzar Rodríguez, Estíbaliz
Seminario Huárriz, Amaia
Díaz Leal, Juan Luis
Alamillo, Josefa M.
Arrese-Igor Sánchez, César
González García, Esther
Acceso abierto / Sarbide irekiainfo:eu-repo/semantics/openAccess
Artículo / Artikuluainfo:eu-repo/semantics/article
Versión publicada / Argitaratu den bertsioainfo:eu-repo/semantics/publishedVersion
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Drought stress is a major factor limiting symbiotic nitrogen fixation (NF) in soybean crop production. However, the regulatory mechanisms involved in this inhibition are still controversial. Soybean plants were symbiotically grown in a split-root system (SRS), which allowed for half of the root system to be irrigated at field capacity while the other half remained water deprived. NF declined in t ... [++]
Drought stress is a major factor limiting symbiotic nitrogen fixation (NF) in soybean crop production. However, the regulatory mechanisms involved in this inhibition are still controversial. Soybean plants were symbiotically grown in a split-root system (SRS), which allowed for half of the root system to be irrigated at field capacity while the other half remained water deprived. NF declined in the water-deprived root system while nitrogenase activity was maintained at control values in the well-watered half. Concomitantly, amino acids and ureides accumulated in the water-deprived belowground organs regardless of transpiration rates. Ureide accumulation was found to be related to the decline in their degradation activities rather than increased biosynthesis. Finally, proteomic analysis suggests that plant carbon metabolism, protein synthesis, amino acid metabolism, and cell growth are among the processes most altered in soybean nodules under drought stress. Results presented here support the hypothesis of a local regulation of NF taking place in soybean and downplay the role of ureides in the inhibition of NF [--]
Drought, Local regulation, N-feedback inhibition, Nitrogen fixation, Proteomics, Soybean, Ureides
Oxford University PressSociety for Experimental Biology
Journal of Experimental Botany, Vol. 64, No. 8, pp. 2171–2182, 2013
Universidad Pública de Navarra. Departamento de Ciencias del Medio Natural / Nafarroako Unibertsitate Publikoa. Natura Ingurunearen Zientziak Saila
Versión del editor
This work was financed by the Spanish Ministry of Economy and Competitiveness (AGL 2011–23738 and AGL 2011-30386-C02-01). EG-Q and AS are holders of PhD fellowships from the Public University of Navarre (735/2008 and 134/2012). EL is a recipient of a Marie Curie International Outgoing Fellowship for Career Development (PIOF-GA-2009–253141).
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