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dc.creatorZulet González, Amaiaes_ES
dc.creatorGil Monreal, Miriames_ES
dc.creatorVillamor, Joji Gracees_ES
dc.creatorZabalza Aznárez, Anaes_ES
dc.creatorHoorn, Renier A.L. van deres_ES
dc.creatorRoyuela Hernando, Mercedeses_ES
dc.date.accessioned2017-04-11T21:19:42Z
dc.date.available2017-04-11T21:19:42Z
dc.date.issued2013
dc.identifier.issn1932-6203 (Electronic)
dc.identifier.urihttp://hdl.handle.net/2454/24093
dc.description.abstractBackground: The herbicides glyphosate (Gly) and imazamox (Imx) inhibit the biosynthesis of aromatic and branched-chain amino acids, respectively. Although these herbicides inhibit different pathways, they have been reported to show several common physiological effects in their modes of action, such as increasing free amino acid contents and decreasing soluble protein contents. To investigate proteolytic activities upon treatment with Gly and Imx, pea plants grown in hydroponic culture were treated with Imx or Gly, and the proteolytic profile of the roots was evaluated through fluorogenic kinetic assays and activity-based protein profiling. Results: Several common changes in proteolytic activity were detected following Gly and Imx treatment. Both herbicides induced the ubiquitin-26 S proteasome system and papain-like cysteine proteases. In contrast, the activities of vacuolar processing enzymes, cysteine proteases and metacaspase 9 were reduced following treatment with both herbicides.Moreover, the activities of several putative serine protease were similarly increased or decreased following treatment with both herbicides. In contrast, an increase in YVADase activity was observed under Imx treatment versus a decrease under Gly treatment. Conclusion: These results suggest that several proteolytic pathways are responsible for protein degradation upon herbicide treatment, although the specific role of each proteolytic activity remains to be determineden
dc.description.sponsorshipA. Zulet and M. Gil-Monreal received funding from Spanish fellowships through the Ministerio de Educación and the Universidad Pública de Navarra, respectively. This work was financially supported through grants from the Spanish Ministry of Education and Science (AGL-2010-18621) and Max Planck Society and the COST Action CM1004 for funding.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.relation.ispartofPLoS ONE 8(9): e73847en
dc.rights© 2013 Zulet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectProteolytic pathwaysen
dc.subjectGlyphosateen
dc.subjectImazamoxen
dc.subjectHerbicidesen
dc.subjectAmino acid biosynthesisen
dc.titleProteolytic pathways induced by herbicides that inhibit amino acid biosynthesisen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Ciencias del Medio Naturales_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Natura Ingurunearen Zientziak Sailaeu
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1371/journal.pone.0073847
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0073847
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen


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© 2013 Zulet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
La licencia del ítem se describe como © 2013 Zulet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.