Anticancer activity of polysaccharides produced by Pleurotus ostreatus in submerged culture
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
It has been known for many years that some compounds produced by edible mushrooms encompass
anticancer activities. Most of production methods were based on cultivation of mushroom in solid
medium. In the present study Pleurotus ostreatus mycelia were grown in submerged culture. The cultivation
of fungal cells in submerged culture resulted in higher growth rate with better control of production ...
[++]
It has been known for many years that some compounds produced by edible mushrooms encompass
anticancer activities. Most of production methods were based on cultivation of mushroom in solid
medium. In the present study Pleurotus ostreatus mycelia were grown in submerged culture. The cultivation
of fungal cells in submerged culture resulted in higher growth rate with better control of production
process. The bioactive polysaccharides (both intracellular and extracellular) were extracted
from culture by solvent repeated precipitation. The polysaccharide structure was determined by examining
NMR, IR spectra and the primary structure of the polysaccharide was mainly glucan. The 13C
NMR spectral pattern indicated the polysaccharides are highly branched with mainly 1→3 and 1→6
linkage. The results of in vitro anti cancer studies demonstrate that this type of polysaccharides possesses
anticancer activity against human oesophageal cancer cell line. Moreover, in the course of in vitro
studies, mushroom polysaccharides showed anti-tumour activity and also considered to be biological
response modifier because of their mechanism of action through stimulation of the immune
system. The polysaccharide activity is especially beneficial in clinics when used as an adjuvant with
chemotherapy to decrease its side effect. This work describes production process of anti cancer compound(
s) by mushrooms and suitable for pharmaceutical industries. [--]
Materias
Pleorotus ostreatus,
Anticancer activity,
Submerged culture,
Extracellular polysaccharides (EPS)
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Comunicación presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.