Ectopic expression of constitutively activated small GTPase cdc42 alters the morphology of haploid and dikaryotic hyphae in the filamentous homobasidiomycete Schizophyllum commune
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
Cloning of the Cdc42 gene from Schizophyllum commune enabled investigation
of the role of ScCdc42 in the regulation of vegetative growth and sexual
reproduction in this fungus, which has a well-characterized hyphal cell structure,
cytoskeleton and mating system. Ectopic expression of the constitutively
active Sccdc42G12V or Sccdc42Q61L allele from native or inducible ScCel1 promoters
had dra ...
[++]
Cloning of the Cdc42 gene from Schizophyllum commune enabled investigation
of the role of ScCdc42 in the regulation of vegetative growth and sexual
reproduction in this fungus, which has a well-characterized hyphal cell structure,
cytoskeleton and mating system. Ectopic expression of the constitutively
active Sccdc42G12V or Sccdc42Q61L allele from native or inducible ScCel1 promoters
had dramatic effects on hyphal morphology, cytoskeletal structure
and Cdc42 localization, while ectopic over-expression of the wt or the dominant
negative ScCdc42D118A allele had no detectable effect. For transformants
with constitutively active Sccdc42 tip growth of apical cells in the leading hyphae
was normal but polar tip growth in side branches was altered implying
different regulation of polarity establishment in the two groups of apical
cells.
The S. commune genome also contains a gene encoding RacGTPase. Rac1
might regulate the polarized growth of leading hyphae while ScCdc42 regulates
the development of side branches in S. commune. In transformants with
constitutively active Sccdc42 branch emergence at exceptional sites and
isotropic growth next to the septum proved that the branch site selection and
subsequent hyphal development are under ScCdc42 control. Poor dikaryotization
along with irregular clamp connections in mates with Sccdc42G12V or
Sccdc42Q61L allele suggested that Cdc42 also contributes to efficient mating in
S. commune. Our results provide strong evidence that Cdc42 is involved in
the control of hyphal morphogenesis in filamentous homobasidiomycetes. [--]
Materias
Schizophyllum commune,
Cdc42 gene
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen de la conferencia presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.