Molecular characterisation and expression analysis of developmentally regulated genes in Agaricus bisporus
Fecha
2006Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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nodoi-noplumx
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Resumen
Analysis of cDNA transcripts, PCR based methods and genomic library
screening have been used to clone and characterise developmentally regulated
genes in the cultivated white button mushroom Agaricus bisporus. Up-regulated
genes identified during the rapid expansion phase of the sporophore
include sugar transporter gene sut1, putative riboflavin-aldehyde-formingenzyme
gene (raf) and three no ...
[++]
Analysis of cDNA transcripts, PCR based methods and genomic library
screening have been used to clone and characterise developmentally regulated
genes in the cultivated white button mushroom Agaricus bisporus. Up-regulated
genes identified during the rapid expansion phase of the sporophore
include sugar transporter gene sut1, putative riboflavin-aldehyde-formingenzyme
gene (raf) and three novel morphogenes mag2-mag4. Further, a hexose
transporter gene sut2 and lectin genes abl1 and abl2, among others have
been cloned from A. bisporus using PCR based strategies. Northern analysis
indicated their up-regulation during sporophore differentiation and development.
Sugar transporter gene sut1 transcripts increased abundantly during
sporophore development and although sut1 showed varying levels of homology
to other sugar transporters, its substrate preference could not be identified
based on homology. Interestingly, analysis of basidiomycete genome sequences
revealed the presence of a putative sut1 homolog in the white rot
fungus Phanerocheate chrysosporium. On the other hand, Ab sut2 showed
strong homology to fungal glucose/hexose transporters and its homologs also
appear to be present in A. bitorquis and Coprinus cinereus suggesting a generic
role. Analysis of the genomic cosmid clones revealed that the lectin genes
abl1 and abl2 are present in close proximity to each other and further characterisation
is on-going. [--]
Materias
Agaricus bisporus,
Developmentally regulated genes
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen del poster presentado al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.