Copper in fruiting body development of Coprinus cinereus
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
The model homobasidiomycete Coprinopsis cinerea grows best at 37°C, but,
normally, it produces fruiting bodies only at moderate temperatures around
25-28°C. Light is needed to induce fruiting and also for fruiting body maturation.
Cultures kept after fruiting induction predominantly in the dark form
structures with an extended stipe and an underdeveloped cap (so-called “etiolated
stipes”). I ...
[++]
The model homobasidiomycete Coprinopsis cinerea grows best at 37°C, but,
normally, it produces fruiting bodies only at moderate temperatures around
25-28°C. Light is needed to induce fruiting and also for fruiting body maturation.
Cultures kept after fruiting induction predominantly in the dark form
structures with an extended stipe and an underdeveloped cap (so-called “etiolated
stipes”). In a day/night rhythm, caps develop further, basidia are
formed, in which karyogamy and meiosis occurs and of which the basidiospores
bud off. Besides light, fruiting body development in basidiomycetes
has been repeatedly linked to enzymes belonging to the group of
phenoloxidases, in particular the multi-copper containing laccases. However,
their roles in fruiting remain unclear.
In attempts to induce laccase production in liquid standing cultures at
37°C, to our surprise we found unusual inititation of fruiting body development.
However, the abundantly formed primordia did never develop into
mature fruiting bodies but into large-sized etiolated stipes, both in dark and
in light. Laccase under these conditions was not detected in the medium but
bound to the fruiting initiating mycelium. Moreover, enzyme production and
etiolated stipe formation correlated with an increase from pH 5.5 to a slightly
alkaline pH. Ammonium was found to be produced and nitrate reductase activity
has enzymatically been shown. Under normal fruiting conditions, addition
of copper to cultures enhances fruiting initiation in time and number.
To further unravel the potential involvement of laccases in fruiting as well
as of proteins influencing ammonia secretion, we are studying expression of
corresponding genes during vegetative growth and fruiting body development.
Work in our laboratory is supported by DBU (Deutsche Bundesstiftung
Umwelt). MNG holds a CONACYT (Mexico) PhD studentship. [--]
Materias
Copper,
Fruiting body development,
Coprinus cinereus
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen del poster presentado al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
Entidades Financiadoras
Work in the author's laboratory is supported by DBU (Deutsche Bundesstiftung Umwelt).