Novel highly sensitive protein sensors based on tapered optical fibres modified with Au-based nanocoatings
Fecha
2016Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1155/2016/8129387
Resumen
Novel protein sensors based on tapered optical fibres modified with Au coatings deposited using two different procedures
are proposed. Au-based coatings are deposited onto a nonadiabatic tapered optical fibre using (i) a novel facile method
composed of layer-by-layer deposition consisting of polycation (poly(allylamine hydrochloride), PAH) and negatively charged SiO2
nanoparticles (NPs) follow ...
[++]
Novel protein sensors based on tapered optical fibres modified with Au coatings deposited using two different procedures
are proposed. Au-based coatings are deposited onto a nonadiabatic tapered optical fibre using (i) a novel facile method
composed of layer-by-layer deposition consisting of polycation (poly(allylamine hydrochloride), PAH) and negatively charged SiO2
nanoparticles (NPs) followed by the deposition of the charged Au NPs and (ii) the sputtering technique. The Au NPs and Au thin
film surfaces are then modified with biotin in order to bind streptavidin (SV) molecules and detect them. The sensing principle is
based on the sensitivity of the transmission spectrum of the device to changes in the refractive index of the coatings induced by the
SV binding to the biotin. Both sensors showed high sensitivity to SV, with the lowest measured concentration levels below 2.5 nM.
The calculated binding constant for the biotin-SV pair was 2.2 × 10−11 M−1 when a tapered fibre modified with the LbL method was
used, with a limit of detection (LoD) of 271 pM. The sensor formed using sputtering had a binding constant of 1.01 × 10−10 M−1
with a LoD of 806 pM. These new structures and their simple fabrication technique could be used to develop other biosensors. [--]
Materias
Novel protein sensors,
Tapered optical fibers,
Au-based coatings
Editor
Hindawi Publishing Corporation
Publicado en
Journal of Sensors, Volume 2016, Article ID 8129387, 11 pages
Departamento
Universidad Pública de Navarra. Departamento de Ingeniería Eléctrica y Electrónica /
Nafarroako Unibertsitate Publikoa. Ingeniaritza Elektrikoa eta Elektronikoa Saila
Versión del editor
Entidades Financiadoras
This work was supported in part by the Spanish Ministry
of Economy and Competitiveness-CICYT-FEDER TEC2013-43679R Research Grant and UPNA predoctoral and mobility
research grants. The authors also acknowledge the support
of UK Engineering and Physical Sciences Research Council
Platform grant (EP/H02252X/1) and responsive mode grant
(EP/L010437/1). The authors would like also to acknowledge
the support of the EPSRC EP/G061661/1 Advanced Ultrasonics
Platform Grant, University of Nottingham, Dean of
Engineering Faculty Award.