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dc.creatorUrra Rodríguez, Marinaes_ES
dc.date.accessioned2018-08-13T08:07:36Z
dc.date.available2023-07-01T23:00:11Z
dc.date.issued2018
dc.identifier.urihttps://hdl.handle.net/2454/29748
dc.description.abstractCrop productivity relies heavily on fertilization. However, the augmented use of fertilizers by industrial farming results in an over-accumulation of nutrients in the soil, which induces nutritional stresses. The utilization of nitrate, ammonium, and urea as nitrogen sources leads to an elevated intracellular accumulation of ammonium, which makes indispensable to understand and elucidate the mechanism by which plants face ammonium toxicity. Early studies on plant metabolism of polyamines pointed to their involvement in responses to different environmental stresses. However, the precise mechanisms by which polyamines control plant responses to stress stimuli have not been elucidated yet. Previous results of our laboratory suggested the importance of the “urea cycle” on the tolerance of Medicago truncatula seedlings to ammonium, in which polyamine catabolism might play a key role in such tolerance, but there are still important gaps to fulfill. The excess of ammonium originated either from ammonium nutrition or from polyamine metabolism may be suggested to act as a feedback inhibitor of amine oxidases since polyamines, mainly putrescine, were accumulated while the content of γaminobutyric acid decreased. In the present study, an intra- and interspecific phylogenetic analyses of amine oxidases are performed in order to characterize them. Moreover, the differential effect of distinct nitrogen nutrition and doses on diamine oxidases and polyamine oxidases activities involved in the catabolic processes of polyamines in connection to the “urea cycle” was assayed. In general terms, the results obtained in this study showed higher diamine oxidase activities in Medicago truncatula plant shoots than that observed in roots. In addition, plants supplied with low dose of ammonium exhibited significantly higher diamine oxidase activity in shoots in comparison with nitrate- and urea-fed plants, whereas diamine oxidase activity was significantly higher in roots of ammonium-fed plants at high dose. Since diamine oxidase activity has been shown to be increased under ammonium conditions in Medicago truncatula tissues, this might suggest the importance of polyamine catabolism in the tolerance against high ammonium conditions. Although alternative mechanisms underlying ammonium stress/tolerance response are proposed in this report, future research should be performed to elucidate the mechanisms underlying plant ammonium tolerance responses.es_ES
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.subjectMedicago truncatulaes_ES
dc.subjectAmine oxidaseses_ES
dc.subjectPolyamineses_ES
dc.subjectDiamine oxidasees_ES
dc.subjectNitrogenes_ES
dc.subjectAmmoniumes_ES
dc.titleAmine oxidases in the oxidative pathway of polyamines in Medicago truncatula grown under different nitrogen sourcesen
dc.typeTrabajo Fin de Grado/Gradu Amaierako Lanaes
dc.typeinfo:eu-repo/semantics/bachelorThesisen
dc.date.updated2018-06-28T12:40:09Z
dc.contributor.affiliationEscuela Técnica Superior de Ingenieros Agrónomoses_ES
dc.contributor.affiliationNekazaritza Ingeniarien Goi Mailako Eskola Teknikoaeu
dc.description.degreeGraduado o Graduada en Ingeniería Agroalimentaria y del Medio Rural por la Universidad Pública de Navarraes_ES
dc.description.degreeNekazaritzako Elikagaien eta Landa Ingurunearen Ingeniaritzan graduatua Nafarroako Unibertsitate Publikoaneu
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.embargo.terms2023-07-01es_ES
dc.contributor.advisorTFEMorán Juez, José Fernandoes_ES
dc.contributor.advisorTFERoyo Castillejo, Beatrizes_ES


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