Non-additive transcriptional profiles underlie dikaryotic superiority in Pleurotus ostreatus laccase activity
Fecha
2013Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.1371/journal.pone.0073282
Resumen
Background: The basidiomycete Pleurotus ostreatus is an efficient producer of laccases, a group of enzymes appreciated for
their use in multiple industrial processes. The aim of this study was to reveal the molecular basis of the superiority of laccase
production by dikaryotic strains compared to their parental monokaryons.
Methodology/Principal Findings: We bred and studied a set of dikaryoti ...
[++]
Background: The basidiomycete Pleurotus ostreatus is an efficient producer of laccases, a group of enzymes appreciated for
their use in multiple industrial processes. The aim of this study was to reveal the molecular basis of the superiority of laccase
production by dikaryotic strains compared to their parental monokaryons.
Methodology/Principal Findings: We bred and studied a set of dikaryotic strains starting from a meiotic population of
monokaryons. We then completely characterised the laccase allelic composition, the laccase gene expression and activity
profiles in the dikaryotic strain N001, in two of its meiotic full-sib monokaryons and in the dikaryon formed from their
mating.
Conclusions/Significance: Our results suggested that the dikaryotic superiority observed in laccase activity was due to nonadditive
transcriptional increases in lacc6 and lacc10 genes. Furthermore, the expression of these genes was divergent in
glucose- vs. lignocellulose-supplemented media and was highly correlated to the detected extracellular laccase activity.
Moreover, the expression profile of lacc2 in the dikaryotic strains was affected by its allelic composition, indicating a
putative single locus heterozygous advantage. [--]
Materias
Pleurotus ostreatus,
Laccases,
Dikaryotic strains
Editor
Public Library of Science
Publicado en
Plos One, 8(9): e73282
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
Versión del editor
Entidades Financiadoras
This work was supported by funds obtained from AGL2011-30495 of the Spanish National Research Plan; Bioethanol-Euroinnova, the Autonomous
Government of Navarre; and by additional institutional support from the Public University of Navarre.