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Promoter methylation of RASSF1A associates to adult secondary glioblastomas and pediatric glioblastomas
dc.creator | Muñoz, Jorge | es_ES |
dc.creator | Inda, María del Mar | es_ES |
dc.creator | Lázcoz, Paula | es_ES |
dc.creator | Zazpe, Idoya | es_ES |
dc.creator | Fan, Xing | es_ES |
dc.creator | Alfaro, Jorge | es_ES |
dc.creator | Tuñón Álvarez, Teresa | es_ES |
dc.creator | Rey, Juan A. | es_ES |
dc.creator | Castresana, Javier S. | es_ES |
dc.date.accessioned | 2018-10-25T06:11:43Z | |
dc.date.available | 2018-10-25T06:11:43Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 2090-5505 (Print) | |
dc.identifier.issn | 2090-5513 (Electronic) | |
dc.identifier.uri | https://hdl.handle.net/2454/31209 | |
dc.description.abstract | While allelic losses and mutations of tumor suppressor genes implicated in the etiology of astrocytoma have been widely assessed, the role of epigenetics is still a matter of study. We analyzed the frequency of promoter hypermethylation by methylation-specific PCR (MSP) in five tumor suppressor genes (PTEN, MGMT, RASSF1A, p14ARF, and p16INK4A), in astrocytoma samples and cell lines. RASSF1A was the most frequently hypermethylated gene in all grades of astrocytoma samples, in cell lines, and in adult secondary GBM. It was followed by MGMT. PTEN showed a slight methylation signal in only one GBM and one pilocytic astrocytoma, and in two cell lines; while p14ARF and p16INK4A did not show any evidence of methylation in primary tumors or cell lines. In pediatric GBM, RASSF1A was again the most frequently altered gene, followed by MGMT; PTEN, p14 and p16 showed no alterations. Lack or reduced expression of RASSF1A in cell lines was correlated with the presence of methylation. RASSF1A promoter hypermethylation might be used as a diagnostic marker for secondary GBM and pediatric GBM. Promoter hypermethylation might not be an important inactivation mechanism in other genes like PTEN, p14ARF and p16INK4A, in which other alterations (mutations, homozygous deletions) are prevalent. | en |
dc.description.sponsorship | P. Lazcoz received a predoctoral fellowship from the Universidad Pública de Navarra, Pamplona, Spain. This research was supported in part by grants from the Departamentos de Salud y de Educación del Gobierno de Navarra, Pamplona; Departamento de Sanidad del Gobierno Vasco, Vitoria; Fondo de Investigación Sanitaria; Fundación Científica de la Asociación Española contra el Cáncer, Madrid, Spain. | en |
dc.format.extent | 10 p. | |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | Hindawi / Wiley | en |
dc.relation.ispartof | ISRN Neurology, Volume 2012, Article ID 576578 | en |
dc.rights | © 2012 Jorge Muñoz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | en |
dc.rights.uri | https://creativecommons.org/licenses/by/3.0/ | |
dc.subject | Glioblastomas | en |
dc.subject | RASSF1A | en |
dc.title | Promoter methylation of RASSF1A associates to adult secondary glioblastomas and pediatric glioblastomas | en |
dc.type | info:eu-repo/semantics/article | en |
dc.type | Artículo / Artikulua | es |
dc.contributor.department | Ciencias de la Salud | es_ES |
dc.contributor.department | Osasun Zientziak | eu |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
dc.identifier.doi | 10.5402/2012/576578 | |
dc.relation.publisherversion | http://dx.doi.org/10.5402/2012/576578 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.type.version | Versión publicada / Argitaratu den bertsioa | es |
dc.contributor.funder | Gobierno de Navarra / Nafarroako Gobernua | es |
dc.contributor.funder | Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa | es |