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dc.creatorMuñoz, Jorgees_ES
dc.creatorInda, María del Mares_ES
dc.creatorLázcoz, Paulaes_ES
dc.creatorZazpe, Idoyaes_ES
dc.creatorFan, Xinges_ES
dc.creatorAlfaro, Jorgees_ES
dc.creatorTuñón Álvarez, Teresaes_ES
dc.creatorRey, Juan A.es_ES
dc.creatorCastresana, Javier S.es_ES
dc.date.accessioned2018-10-25T06:11:43Z
dc.date.available2018-10-25T06:11:43Z
dc.date.issued2012
dc.identifier.issn2090-5505 (Print)
dc.identifier.issn2090-5513 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/31209
dc.description.abstractWhile allelic losses and mutations of tumor suppressor genes implicated in the etiology of astrocytoma have been widely assessed, the role of epigenetics is still a matter of study. We analyzed the frequency of promoter hypermethylation by methylation-specific PCR (MSP) in five tumor suppressor genes (PTEN, MGMT, RASSF1A, p14ARF, and p16INK4A), in astrocytoma samples and cell lines. RASSF1A was the most frequently hypermethylated gene in all grades of astrocytoma samples, in cell lines, and in adult secondary GBM. It was followed by MGMT. PTEN showed a slight methylation signal in only one GBM and one pilocytic astrocytoma, and in two cell lines; while p14ARF and p16INK4A did not show any evidence of methylation in primary tumors or cell lines. In pediatric GBM, RASSF1A was again the most frequently altered gene, followed by MGMT; PTEN, p14 and p16 showed no alterations. Lack or reduced expression of RASSF1A in cell lines was correlated with the presence of methylation. RASSF1A promoter hypermethylation might be used as a diagnostic marker for secondary GBM and pediatric GBM. Promoter hypermethylation might not be an important inactivation mechanism in other genes like PTEN, p14ARF and p16INK4A, in which other alterations (mutations, homozygous deletions) are prevalent.en
dc.description.sponsorshipP. Lazcoz received a predoctoral fellowship from the Universidad Pública de Navarra, Pamplona, Spain. This research was supported in part by grants from the Departamentos de Salud y de Educación del Gobierno de Navarra, Pamplona; Departamento de Sanidad del Gobierno Vasco, Vitoria; Fondo de Investigación Sanitaria; Fundación Científica de la Asociación Española contra el Cáncer, Madrid, Spain.en
dc.format.extent10 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherHindawi / Wileyen
dc.relation.ispartofISRN Neurology, Volume 2012, Article ID 576578en
dc.rights© 2012 Jorge Muñoz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.subjectGlioblastomasen
dc.subjectRASSF1Aen
dc.titlePromoter methylation of RASSF1A associates to adult secondary glioblastomas and pediatric glioblastomasen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentCiencias de la Saludes_ES
dc.contributor.departmentOsasun Zientziakeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.5402/2012/576578
dc.relation.publisherversionhttp://dx.doi.org/10.5402/2012/576578
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernuaes
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes


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© 2012 Jorge Muñoz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
La licencia del ítem se describe como © 2012 Jorge Muñoz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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