Fate of a Pseudomonas savastanoi pv. savastanoi type III secretion system mutant in olive plants (Olea europaea L.)
Fecha
2010Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1128/aem.00133-10
Resumen
Pseudomonas savastanoi pv. savastanoi strain NCPPB 3335 is a model bacterial pathogen for studying the
molecular basis of disease production in woody hosts. We report the sequencing of the hrpS-to-hrpZ region of
NCPPB 3335, which has allowed us to determine the phylogenetic position of this pathogen with respect to
previously sequenced Pseudomonas syringae hrp clusters. In addition, we constructe ...
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Pseudomonas savastanoi pv. savastanoi strain NCPPB 3335 is a model bacterial pathogen for studying the
molecular basis of disease production in woody hosts. We report the sequencing of the hrpS-to-hrpZ region of
NCPPB 3335, which has allowed us to determine the phylogenetic position of this pathogen with respect to
previously sequenced Pseudomonas syringae hrp clusters. In addition, we constructed a mutant of NCPPB 3335,
termed T3, which carries a deletion from the 3 end of the hrpS gene to the 5 end of the hrpZ operon. Despite
its inability to multiply in olive tissues and to induce tumor formation in woody olive plants, P. savastanoi pv.
savastanoi T3 can induce knot formation on young micropropagated olive plants. However, the necrosis and
formation of internal open cavities previously reported in knots induced by the wild-type strain were not
observed in those induced by P. savastanoi pv. savastanoi T3. Tagging of P. savastanoi pv. savastanoi T3 with
green fluorescent protein (GFP) allowed real-time monitoring of its behavior on olive plants. In olive plant
tissues, the wild-type strain formed aggregates that colonized the intercellular spaces and internal cavities of
the hypertrophic knots, while the mutant T3 strain showed a disorganized distribution within the parenchyma
of the knot. Ultrastructural analysis of knot sections revealed the release of extensive outer membrane vesicles
from the bacterial cell surface of the P. savastanoi pv. savastanoi T3 mutant, while the wild-type strain exhibited
very few vesicles. This phenomenon has not been described before for any other bacterial phytopathogen during
host infection. [--]
Materias
Pseudomonas savastanoi pv. Savastanoi,
Olive plants
Editor
American Society for Microbiology
Publicado en
Applied and Environmental Microbiology, June 2010, vol. 76, no. 11, p. 3611–3619
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
Versión del editor
Entidades Financiadoras
This research has been supported by Spanish Plan Nacional I D I
grants AGL2008-55311-CO2-01 and AGL2008-55311-CO2-02, cofinanced
by FEDER, as well as by Integrated Action Spain-Italy
HI01-81 and by Ente Cassa di Risparmio di Firenze (2007.1005 and
2008.1573). I.P.-M. was awarded a Spanish MEC fellowship (AP2002-
3800), and L.L. and I.M.M. were supported by Carlsberg Foundation
(Denmark) grant ANS-1282/20 and the Ramón Areces Foundation
(Spain), respectively.