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dc.creatorPérez Martínez, Isabeles_ES
dc.creatorRodríguez Moreno, Luises_ES
dc.creatorLambertsen, Lottees_ES
dc.creatorMatas Casado, Isabel Maríaes_ES
dc.creatorMurillo Martínez, Jesúses_ES
dc.creatorTegli, Stefaniaes_ES
dc.creatorJiménez, Antonio J.es_ES
dc.creatorRamos, Cayoes_ES
dc.date.accessioned2019-01-10T08:27:52Z
dc.date.available2019-01-10T08:27:52Z
dc.date.issued2010
dc.identifier.issn0099-2240 (Print)
dc.identifier.issn1098-5336 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/31931
dc.description.abstractPseudomonas savastanoi pv. savastanoi strain NCPPB 3335 is a model bacterial pathogen for studying the molecular basis of disease production in woody hosts. We report the sequencing of the hrpS-to-hrpZ region of NCPPB 3335, which has allowed us to determine the phylogenetic position of this pathogen with respect to previously sequenced Pseudomonas syringae hrp clusters. In addition, we constructed a mutant of NCPPB 3335, termed T3, which carries a deletion from the 3 end of the hrpS gene to the 5 end of the hrpZ operon. Despite its inability to multiply in olive tissues and to induce tumor formation in woody olive plants, P. savastanoi pv. savastanoi T3 can induce knot formation on young micropropagated olive plants. However, the necrosis and formation of internal open cavities previously reported in knots induced by the wild-type strain were not observed in those induced by P. savastanoi pv. savastanoi T3. Tagging of P. savastanoi pv. savastanoi T3 with green fluorescent protein (GFP) allowed real-time monitoring of its behavior on olive plants. In olive plant tissues, the wild-type strain formed aggregates that colonized the intercellular spaces and internal cavities of the hypertrophic knots, while the mutant T3 strain showed a disorganized distribution within the parenchyma of the knot. Ultrastructural analysis of knot sections revealed the release of extensive outer membrane vesicles from the bacterial cell surface of the P. savastanoi pv. savastanoi T3 mutant, while the wild-type strain exhibited very few vesicles. This phenomenon has not been described before for any other bacterial phytopathogen during host infection.en
dc.description.sponsorshipThis research has been supported by Spanish Plan Nacional I D I grants AGL2008-55311-CO2-01 and AGL2008-55311-CO2-02, cofinanced by FEDER, as well as by Integrated Action Spain-Italy HI01-81 and by Ente Cassa di Risparmio di Firenze (2007.1005 and 2008.1573). I.P.-M. was awarded a Spanish MEC fellowship (AP2002- 3800), and L.L. and I.M.M. were supported by Carlsberg Foundation (Denmark) grant ANS-1282/20 and the Ramón Areces Foundation (Spain), respectively.en
dc.format.extent9 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiology, June 2010, vol. 76, no. 11, p. 3611–3619en
dc.rights© 2010, American Society for Microbiology. All Rights Reserved.en
dc.subjectPseudomonas savastanoi pv. Savastanoien
dc.subjectOlive plantsen
dc.titleFate of a Pseudomonas savastanoi pv. savastanoi type III secretion system mutant in olive plants (Olea europaea L.)en
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/aem.00133-10
dc.relation.publisherversionhttps://doi.org/10.1128/aem.00133-10
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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