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dc.creatorCastanera Andrés, Raúles_ES
dc.creatorLópez Varas, Leticiaes_ES
dc.creatorPisabarro de Lucas, Gerardoes_ES
dc.creatorRamírez Nasto, Lucíaes_ES
dc.date.accessioned2019-01-10T08:27:53Z
dc.date.available2019-01-10T08:27:53Z
dc.date.issued2015
dc.identifier.issn0099-2240 (Print)
dc.identifier.issn1098-5336 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/31934
dc.description.abstractRecently, the lignin-degrading basidiomycete Pleurotus ostreatus has become a widely used model organism for fungal genomic and transcriptomic analyses. The increasing interest in this species has led to an increasing number of studies analyzing the transcriptional regulation of multigene families that encode extracellular enzymes. Reverse transcription (RT) followed by real-time PCR is the most suitable technique for analyzing the expression of gene sets under multiple culture conditions. In this work, we tested the suitability of 13 candidate genes for their use as reference genes in P. ostreatus time course cultures for enzyme production. We applied three different statistical algorithms and obtained a combination of stable reference genes for optimal normalization of RT-quantitative PCR assays. This reference index can be used for future transcriptomic analyses and validation of transcriptome sequencing or microarray data. Moreover, we analyzed the expression patterns of a laccase and a manganese peroxidase (lacc10 and mnp3, respectively) in lignocellulose and glucose-based media using submerged, semisolid, and solid-state fermentation. By testing different normalization strategies, we demonstrate that the use of nonvalidated reference genes as internal controls leads to biased results and misinterpretations of the biological responses underlying expression changes.en
dc.description.sponsorshipThis work was supported by funds from the AGL2011-30495 project of the Spanish National Research Plan and by additional institutional support from the Public University of Navarre. R.C. holds an FPI Ph.D. studentship.en
dc.format.extent10 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiology, 81:4120 –4129en
dc.rights© 2015, American Society for Microbiology. All Rights Reserved.en
dc.subjectPleurotus ostreatusen
dc.subjectRT-quantitative PCR assaysen
dc.titleValidation of reference genes for transcriptional analyses in Pleurotus ostreatus by using reverse transcription-quantitative PCRen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/aem.00402-15
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/AGL2011-30495
dc.relation.publisherversionhttps://doi.org/10.1128/aem.00402-15
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes


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