Mostrar el registro sencillo del ítem

dc.creatorSimón de Goñi, Oihanees_ES
dc.creatorWilliams, Trevores_ES
dc.creatorPossee, Robert D.es_ES
dc.creatorLópez Ferber, Migueles_ES
dc.creatorCaballero Murillo, Primitivoes_ES
dc.date.accessioned2019-01-10T08:27:55Z
dc.date.available2019-01-10T08:27:55Z
dc.date.issued2009
dc.identifier.issn0099-2240 (Print)
dc.identifier.issn1098-5336 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/31939
dc.description.abstractThe stabilities of the Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) complete genome bacmid (Sfbac) and a deletion recombinant (Sf29null) in which the Sf29 gene was replaced by a kanamycin resistance cassette were determined during sequential rounds of per os infection in insect larvae. The Sf29 gene is a viral factor that determines the number of virions in occlusion bodies (OBs). The Sf29null bacmid virus was able to recover the Sf29 gene during passage. After the third passage (P3) of Sf29null bacmid OBs, the population was observed to reach an equilibrium involving a mixture of those with a kanamycin resistance cassette and those with the Sf29 gene. The biological activity of Sf29null bacmid OBs at P3 was similar to that of Sfbac OBs. The recovered gene in the Sf29null virus was 98 to 100% homologous to the Sf29 genes of different SfMNPV genotypes. Reverse transcription-PCR analysis of uninoculated S. frugiperda larvae confirmed the expression of the SfMNPV ie-0 and Sf29 genes, indicating that the insect colony harbors a covert SfMNPV infection. Additionally, the nonessential bacterial artificial chromosome vector was spontaneously deleted from both viral genomes upon passage in insects.en
dc.description.sponsorshipThis study received financial support from the Spanish Ministry for Science and Technology projects AGL2005-07909-CO3-01 and AGL2008-05456-CO3-01/AGR. O.S. received a Spanish Ministry of Education and Culture postdoctoral fellowship.en
dc.format.extent7 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiology, Feb. 2010, Vol. 76, No. 3, p. 803–809en
dc.rights© 2010, American Society for Microbiology. All Rights Reserved.en
dc.subjectSpodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV)en
dc.subjectSerial passageen
dc.titleStability of a Spodoptera frugiperda nucleopolyhedrovirus deletion recombinant during serial passage in insectsen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Instituto de Agrobiotecnologíaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Agrobioteknologiako Institutuaeu
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/aem.01894-09
dc.relation.publisherversionhttps://doi.org/10.1128/aem.01894-09
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


Ficheros en el ítem

Thumbnail
Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem