A Chrysodeixis chalcites single-nucleocapsid nucleopolyhedrovirus population from the Canary Islands is genotypically structured to maximize survival
Fecha
2013Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1128/aem.02409-13
Resumen
A Chrysodeixis chalcites single-nucleocapsid nucleopolyhedrovirus wild-type isolate from the Canary Islands, Spain, named
ChchSNPV-TF1 (ChchTF1-wt), appears to have great potential as the basis for a biological insecticide for control of the pest. An
improved understanding of the genotypic structure of this wild-type strain population should facilitate the selection of genotypes
for inclusion ...
[++]
A Chrysodeixis chalcites single-nucleocapsid nucleopolyhedrovirus wild-type isolate from the Canary Islands, Spain, named
ChchSNPV-TF1 (ChchTF1-wt), appears to have great potential as the basis for a biological insecticide for control of the pest. An
improved understanding of the genotypic structure of this wild-type strain population should facilitate the selection of genotypes
for inclusion in a bioinsecticidal product. Eight genetically distinct genotypes were cloned in vitro: ChchTF1-A to
ChchTF1-H. Quantitative real-time PCR (qPCR) analysis confirmed that ChchTF1-A accounted for 36% of the genotypes in the
wild-type population. In bioassays, ChchTF1-wt occlusion bodies (OBs) were significantly more pathogenic than any of the component
single-genotype OBs, indicating that genotype interactions were likely responsible for the pathogenicity phenotype of
wild-type OBs. However, the wild-type population was slower killing and produced higher OB yields than any of the single genotypes
alone. These results strongly suggested that the ChchTF1-wt population is structured to maximize its transmission efficiency.
Experimental OB mixtures and cooccluded genotype mixtures containing the most abundant and the rarest genotypes, at
frequencies similar to those at which they were isolated, revealed a mutualistic interaction that restored the pathogenicity of
OBs. In OB and cooccluded mixtures containing only the most abundant genotypes, ChchTF1-ABC, OB pathogenicity was even
greater than that of wild-type OBs. The ChchTF1-ABC cooccluded mixture killed larvae 33 h faster than the wild-type population
and remained genotypically and biologically stable throughout five successive passages in vivo. In conclusion, the ChchTF1-
ABC mixture shows great potential as the active ingredient of a bioinsecticide to control C. chalcites in the Canary Islands. [--]
Materias
ChchSNPV-TF1,
Genotypic structure,
Canary islands
Editor
American Society for Microbiology
Publicado en
Applied and Environmental Microbiology, December 2013, Volume 79, Number 24, p. 7709–7718
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
Versión del editor
Entidades Financiadoras
This study received financial support from the Instituto Nacional de
Investigaciones Agrarias (project RTA2010-00016-C2-02), from the Programa
Nacional de España de I D i (project AGL2008-05456-CO3-01/
AGR), and from the Gobierno de Navarra (project IIQ14065:RI1).