Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures
Fecha
2012Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.1128/aem.07880-11
Resumen
The genome of the white rot basidiomycete Pleurotus ostreatus includes 12 phenol oxidase (laccase) genes. In this study, we examined
their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures)
and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription-
quantitati ...
[++]
The genome of the white rot basidiomycete Pleurotus ostreatus includes 12 phenol oxidase (laccase) genes. In this study, we examined
their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures)
and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription-
quantitative PCR (RT-qPCR)-based approach and focused on determining the reaction parameters (in particular, the
reference gene set for the normalization and reaction efficiency determinations) used to achieve an accurate estimation of the
relative gene expression values. The results suggested that (i) laccase gene transcription is upregulated in the induced submerged
fermentation (iSmF) cultures but downregulated in the solid fermentation (SSF) cultures, (ii) the Lacc2 and Lacc10 genes are the
main sources of laccase activity in the iSmF cultures upon induction with water-soluble wheat straw extracts, and (iii) an additional,
as-yet-uncharacterized activity (Unk1) is specifically induced in SSF cultures that complements the activity of Lacc2 and
Lacc10. Moreover, both the enzymatic laccase activities and the Lacc gene family transcription profiles greatly differ between
closely related strains. These differences can be targeted for biotechnological breeding programs for enzyme production in submerged
fermentation reactors. [--]
Materias
Pleurotus ostreatus,
Submerged fermentation,
Solid fermentation,
Laccase gene transcription
Editor
American Society for Microbiology
Publicado en
Applied and Environmental Microbiology, June 2012 Volume 78 Number 11, p. 4037–4045
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
Versión del editor
Entidades Financiadoras
This work was supported by funds from project AGL2008-05608-C02-01
of the Spanish National Research Plan, from Bioethanol-Euroinnova, and
from the Autonomous Government of Navarre and by additional institutional
support from the Public University of Navarre.