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dc.creatorCastanera Andrés, Raúles_ES
dc.creatorPérez Garrido, Gumersindaes_ES
dc.creatorOmarini, Alejandraes_ES
dc.creatorAlfaro Sánchez, Manueles_ES
dc.creatorPisabarro de Lucas, Gerardoes_ES
dc.creatorFaraco, Vicenzaes_ES
dc.creatorAmore, Antonellaes_ES
dc.creatorRamírez Nasto, Lucíaes_ES
dc.date.accessioned2019-01-10T08:27:58Z
dc.date.available2019-01-10T08:27:58Z
dc.date.issued2012
dc.identifier.issn0099-2240 (Print)
dc.identifier.issn1098-5336 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/31946
dc.description.abstractThe genome of the white rot basidiomycete Pleurotus ostreatus includes 12 phenol oxidase (laccase) genes. In this study, we examined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription- quantitative PCR (RT-qPCR)-based approach and focused on determining the reaction parameters (in particular, the reference gene set for the normalization and reaction efficiency determinations) used to achieve an accurate estimation of the relative gene expression values. The results suggested that (i) laccase gene transcription is upregulated in the induced submerged fermentation (iSmF) cultures but downregulated in the solid fermentation (SSF) cultures, (ii) the Lacc2 and Lacc10 genes are the main sources of laccase activity in the iSmF cultures upon induction with water-soluble wheat straw extracts, and (iii) an additional, as-yet-uncharacterized activity (Unk1) is specifically induced in SSF cultures that complements the activity of Lacc2 and Lacc10. Moreover, both the enzymatic laccase activities and the Lacc gene family transcription profiles greatly differ between closely related strains. These differences can be targeted for biotechnological breeding programs for enzyme production in submerged fermentation reactors.en
dc.description.sponsorshipThis work was supported by funds from project AGL2008-05608-C02-01 of the Spanish National Research Plan, from Bioethanol-Euroinnova, and from the Autonomous Government of Navarre and by additional institutional support from the Public University of Navarre.en
dc.format.extent9 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiology, June 2012 Volume 78 Number 11, p. 4037–4045en
dc.rights© 2012, American Society for Microbiology. All Rights Reserved.en
dc.subjectPleurotus ostreatusen
dc.subjectSubmerged fermentationen
dc.subjectSolid fermentationen
dc.subjectLaccase gene transcriptionen
dc.titleTranscriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation culturesen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/aem.07880-11
dc.relation.publisherversionhttps://doi.org/10.1128/aem.07880-11
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernuaes
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes


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