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dc.creatorSundin, George W.es_ES
dc.creatorJacobs, Janette L.es_ES
dc.creatorMurillo Martínez, Jesúses_ES
dc.date.accessioned2019-01-10T08:27:58Z
dc.date.available2019-01-10T08:27:58Z
dc.date.issued2000
dc.identifier.issn0099-2240 (Print)
dc.identifier.issn1098-5336 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/31947
dc.description.abstractThe rulAB locus confers tolerance to UV radiation and is borne on plasmids of the pPT23A family in Pseudomonas syringae. We sequenced 14 rulA alleles from P. syringae strains representing seven pathovars and found sequence differences of 1 to 12% within pathovar syringae, and up to 15% differences between pathovars. Since the sequence variation within rulA was similar to that of P. syringae chromosomal alleles, we hypothesized that rulAB has evolved over a long time period in P. syringae. A phylogenetic analysis of the deduced amino acid sequences of rulA resulted in seven clusters. Strains from the same plant host grouped together in three cases; however, strains from different pathovars grouped together in two cases. In particular, the rulA alleles from P. syringae pv. lachrymans and P. syringae pv. pisi were grouped but were clearly distinct from the other sequenced alleles, suggesting the possibility of a recent interpathovar transfer. We constructed chimeric rulAB expression clones and found that the observed sequence differences resulted in significant differences in UV (wavelength) radiation sensitivity. Our results suggest that specific amino acid changes in RulA could alter UV radiation tolerance and the competitiveness of the P. syringae host in the phyllosphere.en
dc.description.sponsorshipThis work was supported by the U.S. Department of Agriculture (NRICGP 9702832 and NRICGP 1999-02518) and the Texas Agricultural Experiment Station.en
dc.format.extent7 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiology, Dec. 2000 Vol. 66 No. 12, p. 5167–5173en
dc.rights© 2000, American Society for Microbiology. All Rights Reserved.en
dc.subjectrulABen
dc.subjectPseudomonas syringaeen
dc.subjectUV radiation toleranceen
dc.titleSequence diversity of rulA among natural isolates of Pseudomonas syringae and effect on function of rulAB-mediated UV radiation toleranceen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Instituto de Agrobiotecnologíaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Agrobioteknologiako Institutuaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/aem.66.12.5167-5173.2000
dc.relation.publisherversionhttps://doi.org/10.1128/aem.66.12.5167-5173.2000
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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