Genetic linkage map of the edible basidiomycete Pleurotus ostreatus
Fecha
2000Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1128/aem.66.12.5290-5300.2000
Resumen
We have constructed a genetic linkage map of the edible basidiomycete Pleurotus ostreatus (var. Florida). The
map is based on the segregation of 178 random amplified polymorphic DNA and 23 restriction fragment length
polymorphism markers; four hydrophobin, two laccase, and two manganese peroxidase genes; both mating type
loci; one isozyme locus (est1); the rRNA gene sequence; and a repetitive DNA ...
[++]
We have constructed a genetic linkage map of the edible basidiomycete Pleurotus ostreatus (var. Florida). The
map is based on the segregation of 178 random amplified polymorphic DNA and 23 restriction fragment length
polymorphism markers; four hydrophobin, two laccase, and two manganese peroxidase genes; both mating type
loci; one isozyme locus (est1); the rRNA gene sequence; and a repetitive DNA sequence in a population of 80
sibling monokaryons. The map identifies 11 linkage groups corresponding to the chromosomes of P. ostreatus,
and it has a total length of 1,000.7 centimorgans (cM) with an average of 35.1 kbp/cM. The map shows a high
correlation (0.76) between physical and genetic chromosome sizes. The number of crossovers observed per
chromosome per individual cell is 0.89. This map covers nearly the whole genome of P. ostreatus. [--]
Materias
Pleurotus ostreatus,
Genetic linkage maps
Editor
American Society for Microbiology
Publicado en
Applied and Environmental Microbiology, Dec. 2000 Vol. 66, No. 12, p. 5290–5300
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
Versión del editor
Entidades Financiadoras
This work was supported by research projects BIO94-0443 and
BIO99-0278 of the Comisión Nacional de Ciencia y Tecnología and by
funds from the Universidad Pública de Navarra (Pamplona, Spain).
L.M.L. and G.P. hold grants from the Departamento de Educación del
Gobierno de Navarra and the Departamento de Industria del Gobierno
de Navarra, respectively.