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dc.creatorLens, Piet N. L.es_ES
dc.creatorGastesi Barasoain, Rakeles_ES
dc.creatorVergeldt, Frankes_ES
dc.creatorAelst, Adriaan C. vanes_ES
dc.creatorPisabarro de Lucas, Gerardoes_ES
dc.creatorAs, Henk vanes_ES
dc.date.accessioned2019-01-10T08:28:00Z
dc.date.available2019-01-10T08:28:00Z
dc.date.issued2003
dc.identifier.issn0099-2240 (Print)
dc.identifier.issn1098-5336 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/31952
dc.description.abstractThe diffusive properties of anaerobic methanogenic and sulfidogenic aggregates present in wastewater treatment bioreactors were studied using diffusion analysis by relaxation time-separated pulsed-field gradient nuclear magnetic resonance (NMR) spectroscopy and NMR imaging. NMR spectroscopy measurements were performed at 22°C with 10 ml of granular sludge at a magnetic field strength of 0.5 T (20 MHz resonance frequency for protons). Self-diffusion coefficients of H2O in the investigated series of mesophilic aggregates were found to be 51 to 78% lower than the self-diffusion coefficient of free water. Interestingly, self-diffusion coefficients of H2O were independent of the aggregate size for the size fractions investigated. Diffusional transport occurred faster in aggregates growing under nutrient-rich conditions (e.g., the bottom of a reactor) or at high (55°C) temperatures than in aggregates cultivated in nutrient-poor conditions or at low (10°C) temperatures. Exposure of aggregates to 2.5% glutaraldehyde or heat (70 or 90°C for 30 min) modified the diffusional transport up to 20%. In contrast, deactivation of aggregates by HgCl2 did not affect the H2O self-diffusion coefficient in aggregates. Analysis of NMR images of a single aggregate shows that methanogenic aggregates possess a spin-spin relaxation time and self-diffusion coefficient distribution, which are due to both physical (porosity) and chemical (metal sulfide precipitates) factors.en
dc.description.sponsorshipThis research was supported by a TMR Marie Curie fellowship (ERBFMBICT950250) and the Human Capital and Mobility EU Large Scale Facility, Wageningen NMR Centre (ERBCHGECT940061).en
dc.format.extent6 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiology, Nov. 2003, Vol. 69, No. 11, p. 6644–6649en
dc.rights© 2003, American Society for Microbiology. All Rights Reserved.en
dc.subjectNuclear magnetic resonance (NMR)en
dc.subjectDiffusional propertiesen
dc.subjectMethanogenic granular sludgeen
dc.titleDiffusional properties of methanogenic granular sludge: 1H NMR characterizationen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/aem.69.11.6644-6649.2003
dc.relation.publisherversionhttps://doi.org/10.1128/aem.69.11.6644-6649.2003
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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