Sf29 Gene of Spodoptera frugiperda multiple nucleopolyhedrovirus is a viral factor that determines the number of virions in occlusion bodies
Fecha
2008Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.1128/jvi.00099-08
Resumen
The genome of Spodoptera frugiperda multiple nucleopolyhedrovirus (NPV) was inserted into a bacmid
(Sfbac) and used to produce a mutant lacking open reading frame 29 (Sf29null). Sf29null bacmid DNA was
able to generate an infection in S. frugiperda. Approximately six times less DNA was present in occlusion bodies
(OBs) produced by the Sf29null bacmid in comparison to viruses containing this ge ...
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The genome of Spodoptera frugiperda multiple nucleopolyhedrovirus (NPV) was inserted into a bacmid
(Sfbac) and used to produce a mutant lacking open reading frame 29 (Sf29null). Sf29null bacmid DNA was
able to generate an infection in S. frugiperda. Approximately six times less DNA was present in occlusion bodies
(OBs) produced by the Sf29null bacmid in comparison to viruses containing this gene. This reduction in DNA
content was consistent with fewer virus particles being packaged within Sf29null bacmid OBs, as determined
by fractionation of dissolved polyhedra and comparison of occlusion-derived virus (ODV) infectivity in cell
culture. DNA from Sfbac, Sf29null, or Sf29null-repair, in which the gene deletion had been repaired, were
equally infectious when used to transfect S. frugiperda. All three viruses produced similar numbers of OBs,
although those from Sf29null were 10-fold less infectious than viruses with the gene. Insects infected with
Sf29null bacmid died 24 h later than positive controls, consistent with the reduced virus particle content of
Sf29null OBs. Transcripts from Sf29 were detected in infected insects 12 h prior to those from the polyhedrin
gene. Homologs to Sf29 were present in other group II NPVs, and similar sequences were present in entomopoxviruses.
Analysis of the Sf29 predicted protein sequence revealed signal peptide and transmembrane
domains, but the presence of 12 potential N-glycosylation sites suggest that it is not an ODV envelope protein.
Other motifs, including zinc-binding and threonine-rich regions, suggest degradation and adhesion functions.
We conclude that Sf29 is a viral factor that determines the number of ODVs occluded in each OB. [--]
Materias
Spodoptera frugiperda,
Nucleopolyhedroviruses,
Sf29,
Occlusion bodies,
Virions
Editor
American Society for Microbiology
Publicado en
Journal of Virology, Aug. 2008, Vol. 82, No. 16, p. 7897–7904
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This work was supported by core CEH funds. O.S. received a Spanish
Ministry of Education and Culture postdoctoral fellowship.