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dc.creatorSimón de Goñi, Oihanees_ES
dc.creatorWilliams, Trevores_ES
dc.creatorAsensio, Aarón C.es_ES
dc.creatorRos Terés, Sarhayes_ES
dc.creatorGaya Cacho, Andreaes_ES
dc.creatorCaballero Murillo, Primitivoes_ES
dc.creatorPossee, Robert D.es_ES
dc.date.accessioned2019-01-21T13:24:47Z
dc.date.available2019-01-21T13:24:47Z
dc.date.issued2008
dc.identifier.issn0022-538X (Print)
dc.identifier.issn1098-5514 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/32034
dc.description.abstractThe genome of Spodoptera frugiperda multiple nucleopolyhedrovirus (NPV) was inserted into a bacmid (Sfbac) and used to produce a mutant lacking open reading frame 29 (Sf29null). Sf29null bacmid DNA was able to generate an infection in S. frugiperda. Approximately six times less DNA was present in occlusion bodies (OBs) produced by the Sf29null bacmid in comparison to viruses containing this gene. This reduction in DNA content was consistent with fewer virus particles being packaged within Sf29null bacmid OBs, as determined by fractionation of dissolved polyhedra and comparison of occlusion-derived virus (ODV) infectivity in cell culture. DNA from Sfbac, Sf29null, or Sf29null-repair, in which the gene deletion had been repaired, were equally infectious when used to transfect S. frugiperda. All three viruses produced similar numbers of OBs, although those from Sf29null were 10-fold less infectious than viruses with the gene. Insects infected with Sf29null bacmid died 24 h later than positive controls, consistent with the reduced virus particle content of Sf29null OBs. Transcripts from Sf29 were detected in infected insects 12 h prior to those from the polyhedrin gene. Homologs to Sf29 were present in other group II NPVs, and similar sequences were present in entomopoxviruses. Analysis of the Sf29 predicted protein sequence revealed signal peptide and transmembrane domains, but the presence of 12 potential N-glycosylation sites suggest that it is not an ODV envelope protein. Other motifs, including zinc-binding and threonine-rich regions, suggest degradation and adhesion functions. We conclude that Sf29 is a viral factor that determines the number of ODVs occluded in each OB.en
dc.description.sponsorshipThis work was supported by core CEH funds. O.S. received a Spanish Ministry of Education and Culture postdoctoral fellowship.en
dc.format.extent8 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofJournal of Virology, Aug. 2008, Vol. 82, No. 16, p. 7897–7904en
dc.rights© 2008, American Society for Microbiology. All Rights Reserved.en
dc.subjectSpodoptera frugiperdaen
dc.subjectNucleopolyhedrovirusesen
dc.subjectSf29en
dc.subjectOcclusion bodiesen
dc.subjectVirionsen
dc.titleSf29 Gene of Spodoptera frugiperda multiple nucleopolyhedrovirus is a viral factor that determines the number of virions in occlusion bodiesen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Instituto de Agrobiotecnologíaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Agrobioteknologiako Institutuaeu
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/jvi.00099-08
dc.relation.publisherversionhttps://doi.org/10.1128/jvi.00099-08
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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