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dc.creatorArrizubieta, M. J.es_ES
dc.creatorToledo Arana, Alejandroes_ES
dc.creatorAmorena Zabalza, Beatrizes_ES
dc.creatorPenadés, José R.es_ES
dc.creatorLasa Uzcudun, Íñigoes_ES
dc.date.accessioned2019-01-23T11:16:25Z
dc.date.available2019-01-23T11:16:25Z
dc.date.issued2004
dc.identifier.issn0021-9193(Print)
dc.identifier.issn1098-5530 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/32093
dc.description.abstractBap (biofilm-associated protein) is a 254-kDa staphylococcal surface protein implicated in formation of biofilms by staphylococci isolated from chronic mastitis infections. The presence of potential EF-hand motifs in the amino acid sequence of Bap prompted us to investigate the effect of calcium on the multicellular behavior of Bap-expressing staphylococci. We found that addition of millimolar amounts of calcium to the growth media inhibited intercellular adhesion of and biofilm formation by Bap-positive strain V329. Addition of manganese, but not addition of magnesium, also inhibited biofilm formation, whereas bacterial aggregation in liquid media was greatly enhanced by metal-chelating agents. In contrast, calcium or chelating agents had virtually no effect on the aggregation of Bap-deficient strain M556. The biofilm elicited by insertion of bap into the chromosome of a biofilm-negative strain exhibited a similar dependence on the calcium concentration, indicating that the observed calcium inhibition was an inherent property of the Bap-mediated biofilms. Site-directed mutagenesis of two of the putative EF-hand domains resulted in a mutant strain that was capable of forming a biofilm but whose biofilm was not inhibited by calcium. Our results indicate that Bap binds Ca2+ with low affinity and that Ca2+ binding renders the protein noncompetent for biofilm formation and for intercellular adhesion. The fact that calcium inhibition of Bap-mediated multicellular behavior takes place in vitro at concentrations similar to those found in milk serum supports the possibility that this inhibition is relevant to the pathogenesis and/or epidemiology of the bacteria in the mastitis process.en
dc.description.sponsorshipThis work was supported by the Comisión Interministerial de Ciencia y Tecnología of Spain through grant BIO2002-01841 to M.J.A. and grant BIO2002-04542-C02 to I.L. and J.P. and by the Beca Ortiz de Landazuri award from the Departamento de Salud del Gobierno de Navarra. A.T.-A. is a predoctoral fellow of the Ministerio de Educación, Cultura y Deporte (FPU), Spain.en
dc.format.extent9 p.
dc.format.mimetypeapplication/pdfen
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofJournal of Bacteriology, vol. 186, nº 22, nov. 2004, p. 7490–7498en
dc.rights© 2004, American Society for Microbiology. All Rights Reserved.en
dc.subjectBap (biofilm-associated protein)en
dc.subjectStaphylococcus aureusen
dc.subjectFormation of biofilmsen
dc.titleCalcium inhibits bap-dependent multicellular behavior in Staphylococcus aureusen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentIdAB – Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1128/jb.186.22.7490-7498.2004
dc.relation.publisherversionhttps://doi.org/10.1128/jb.186.22.7490-7498.2004
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernuaes


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