Listar por autor UPNA "Farrán Blanch, Inmaculada"
Mostrando ítems 1-20 de 21
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A chloroplast-derived Toxoplasma gondiiGRA4 antigen used as an oral vaccine protects against toxoplasmosis in mice
The parasitic protozoan Toxoplasma gondii, the causal agent of toxoplasmosis, can infect most mammals and birds. In human medicine, T. gondii can cause complications in pregnant women and immunodeficient individuals, while ... -
Estudio de las bases moleculares de la infección respiratoria por el patógeno bacteriano Haemophilus influenzae: efecto de la auxotrofía metabólica e implementación de un nuevo modelo experimental preclínico
Este trabajo de tesis doctoral se centra en el estudio de las bases moleculares de la infección respiratoria por la bacteria Haemophilus influenzae, miembro del microbioma respiratorio humano y patógeno oportunista de ... -
The extradomain a of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice
The Extradomain A from fibronectin (EDA) has an immunomodulatory role as fusion protein with viral and tumor antigens, but its effect when administered with bacteria has not been assessed. Here, we investigated the adjuvant ... -
Functional improvement of human cardiotrophin 1 produced in tobacco chloroplasts by co-expression with plastid thioredoxin m
Human cardiotrophin 1 (CT1), a cytokine with excellent therapeutic potential, was previously expressed in tobacco chloroplasts. However, the growth conditions required to reach the highest expression levels resulted in an ... -
The fusion of Toxoplasma gondii SAG1 vaccine candidate to Leishmania infantum heat shock protein 83-kDa improves expression levels in tobacco chloroplasts
Chloroplast transformation technology has emerged as an alternative platform offering many advantages over nuclear transformation. SAG1 is the main surface antigen of the intracellular parasite Toxoplasma gondii and a ... -
Heat treatment alleviates the growth and photosynthetic impairment of transplastomic plants expressing Leishmania infantum Hsp83-Toxoplasma gondii SAG1 fusion protein
Previously, we showed that transplastomic tobacco plants expressing the LiHsp83-SAG1 fusion protein displayed a chlorotic phenotype and growth retardation, while plants expressing the SAG1 and GRA4 antigens alone did not. ... -
High-density seedling expression system for the production of bioactive human cardiotrophin-1, a potential therapeutic cytokine, in transgenic tobacco chloroplasts
Histidine‐tagged human cardiotrophin‐1 (hCT‐1), a recently discovered cytokine with excellent therapeutic potential, was expressed in tobacco chloroplasts under the transcriptional and translational control of two different ... -
Increased bioethanol production from commercial tobacco cultivars overexpressing thioredoxin f grown under field conditions
Bioethanol is mainly produced from food crops such as sugar cane and maize while it has been held partly responsible for the rise of food commodity prices. Tobacco, integrated in biorefinery facilities for the extraction ... -
Influencia de la fertilización nitrogenada y la conservación post-cosecha sobre las proteínas del gluten la calidad harinopanadera del trigo blando
Las proteínas del gluten, compuestas por gliadinas y gluteninas, son las responsables de que la masa elaborada con harina de trigo posea unas propiedades viscoelásticas que la distinguen con respecto a la de otros cereales. ... -
NTRC and thioredoxin f overexpression differentially induces starch accumulation in tobacco leaves
Thioredoxin (Trx) f and NADPH-dependent Trx reductase C (NTRC) have both been proposed as major redox regulators of starch metabolism in chloroplasts. However, little is known regarding the specific role of each protein ... -
Overexpression of plastidial thioredoxin f leads to enhanced starch accumulation in tobacco leaves
Starch, the most abundant storage carbohydrate in plants, has been a major feedstock for first‐generation biofuels. Growing fuel demands require, however, that the starch yields of energy crops be improved. Leaf starch is ... -
Overexpression of plastidial thioredoxins f and m differentially alters photosynthetic activity and response to oxidative stress in tobacco plants
Plants display a remarkable diversity of thioredoxins (Trxs), reductases controlling the thiol redox status of proteins. The physiological function of many of them remains elusive, particularly for plastidial Trxs f and ... -
Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and ... -
Overexpression of thioredoxin m in tobacco chloroplasts inhibits the protein kinase STN7 and alters photosynthetic performance
The activity of the protein kinase STN7, involved in phosphorylation of the light-harvesting complex II (LHCII) proteins, has been reported as being co-operatively regulated by the redox state of the plastoquinone pool ... -
Papel de las tiorredoxinas plastidiales en la regulación del metabolismo del cloroplasto
Carbon (C) and nitrogen (N) are two essential nutrients required for plant growth and development. The cellular metabolism of both elements is tightly coordinated and subjected to a complex regulation. In this regard, the ... -
Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranes
The leaf mesophyll CO2 conductance and the concentration of CO2 within the chloroplast are major factors affecting photosynthetic performance. Previous studies have shown that the aquaporin NtAQP1 (which localizes to the ... -
Plant growth-promoting traits of yeasts isolated from Spanish vineyards: benefits for seedling development
It is known that some microorganisms can enhance plant development. However, the use of yeasts as growth-promoting agents has been poorly investigated. The aim of this study was the characterisation of a collection of 69 ... -
Post-harvest light treatment increases expression levels of recombinant proteins in transformed plastids of potato tubers
Plastid genetic engineering represents an attractive system for the production of foreign proteins in plants. Although high expression levels can be achieved in leaf chloroplasts, the results for non-photosynthetic plastids ... -
Regulation of the response of plants to volatile compounds emitted by fungal phytopathogens
El crecimiento y el desarrollo de las plantas están afectados por microorganismos presentes en la filosfera, la rizosfera y/o la endosfera. En la fase de precolonización, antes de que se establezca un contacto físico con ... -
Successful biocontrol of major postharvest and soil-borne plant pathogenic fungi by antagonistic yeasts
Fungal pathogens are the main biotic burden of productivity for economically important crops under field, greenhouse or postharvest conditions. The discovery and development of new environmental-friendly solutions, such ...