Now showing items 1-2 of 2

    • Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR 

      Lorente, Aiala; Mueller, Wolf; Urdangarín, Edurne; Lázcoz, Paula Upna; Deimling, Andreas von; Sáez Castresana, Javier (BioMed Central, 2008)   Artículo / Artikulua  OpenAccess
      Background: We present two melting curve analysis (MCA)-based semiquantitative real time PCR techniques to detect the promoter methylation status of genes. The first, MCA-MSP, follows the same principle as standard MSP ...
    • Frequent promoter hypermethylation of RASSF1A and CASP8 in neuroblastoma 

      Lázcoz, Paula Upna; Muñoz, Jorge; Nistal, Manuel; Pestaña, Ángel; Encío Martínez, Ignacio Upna; Sáez Castresana, Javier (BioMed Central, 2006)   Artículo / Artikulua  OpenAccess
      Background: Epigenetic alterations and loss of heterozygosity are mechanisms of tumor suppressor gene inactivation. A new carcinogenic pathway, targeting the RAS effectors has recently been documented. RASSF1A, on 3p21.3, ...