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Brucella abortus ornithine lipids are dispensable outer membrane components devoid of a marked pathogen-associated molecular pattern
dc.creator | Palacios Chaves, Leyre | es_ES |
dc.creator | Conde Álvarez, Raquel | es_ES |
dc.creator | Gil Ramírez, Yolanda | es_ES |
dc.creator | Zúñiga Ripa, Amaia | es_ES |
dc.creator | Barquero-Calvo, Elías | es_ES |
dc.creator | Chacón Díaz, Carlos | es_ES |
dc.creator | Chaves Olarte, Esteban | es_ES |
dc.creator | Arce Gorvel, Vilma | es_ES |
dc.creator | Gorvel, Jean-Pierre | es_ES |
dc.creator | Moreno, Edgardo | es_ES |
dc.creator | Miguel, María Jesús de | es_ES |
dc.creator | Grilló Dolset, María Jesús | es_ES |
dc.creator | Moriyón Uría, Ignacio | es_ES |
dc.creator | Iriarte, Maite | es_ES |
dc.date.accessioned | 2014-06-12T11:21:12Z | |
dc.date.available | 2014-06-12T11:21:12Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 1932-6203 (electronic) | |
dc.identifier.other | 595 | |
dc.identifier.uri | https://hdl.handle.net/2454/10827 | |
dc.description.abstract | The brucellae are alpha-Proteobacteria facultative intracellular parasites that cause an important zoonosis. These bacteria escape early detection by innate immunity, an ability associated to the absence of marked pathogen-associated molecular patterns in the cell envelope lipopolysaccharide, lipoproteins and flagellin. We show here that, in contrast to the outer membrane ornithine lipids (OL) of other Gram negative bacteria, Brucella abortus OL lack a marked pathogen-associated molecular pattern activity. We identified two OL genes (olsB and olsA) and by generating the corresponding mutants found that olsB deficient B. abortus did not synthesize OL or their lyso-OL precursors. Liposomes constructed with B. abortus OL did not trigger IL-6 or TNF-alpha release by macrophages whereas those constructed with Bordetella pertussis OL and the olsB mutant lipids as carriers were highly active. The OL deficiency in the olsB mutant did not promote proinflammatory responses or generated attenuation in mice. In addition, OL deficiency did not increase sensitivity to polymyxins, normal serum or complement consumption, or alter the permeability to antibiotics and dyes. Taken together, these observations indicate that OL have become dispensable in the extant brucellae and are consistent within the trend observed in alpha-Proteobacteria animal pathogens to reduce and eventually eliminate the envelope components susceptible of recognition by innate immunity. | en |
dc.description.sponsorship | Research at the laboratories of the authors is supported by the Ministerio de Ciencia y Tecnología of Spain (AGL2008-04514-C03); FIDA, Universidad Nacional de Costa Rica; FS-Conare UNA/UCR IFEG29 Costa Rica; NeTropica P00059 and F00013-02; MICIT/CONICIT IFDG12; Fundación CRUSA-CSIC (CR2008-0006); Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale and the Ministry of Education in France. Fellowship support for L.P.-C., Y.G.-R. and A.Z.-R from the Ministerio de Ciencia y Tecnología of Spain, Gobierno de Navarra and Friends of the University of Navarra is also acknowledged. | en |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | Public Library of Science | en |
dc.relation.ispartof | Plos One, 2011, 6(1): e16030 | en |
dc.rights | © 2011 Palacios-Chaves et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/ | |
dc.subject | Bactericidal cationic peptides | en |
dc.subject | Pseudomonas fluorescens | en |
dc.subject | Lipopolysaccharide | en |
dc.subject | Resistance | en |
dc.subject | Mutants | en |
dc.subject | Cells | en |
dc.subject | Mice | en |
dc.subject | Phosphatidylcholine | en |
dc.subject | Flavobacterium | en |
dc.subject | Identification | en |
dc.title | Brucella abortus ornithine lipids are dispensable outer membrane components devoid of a marked pathogen-associated molecular pattern | en |
dc.type | Artículo / Artikulua | es |
dc.type | info:eu-repo/semantics/article | en |
dc.contributor.department | IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua | es |
dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
dc.identifier.doi | 10.1371/journal.pone.0016030 | |
dc.relation.publisherversion | https://dx.doi.org/10.1371/journal.pone.0016030 | |
dc.type.version | Versión publicada / Argitaratu den bertsioa | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
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La licencia del ítem se describe como © 2011 Palacios-Chaves et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.