Identification of the ovine mannose receptor and its possible role in Visna/Maedi virus infection
Reina Arias, Ramsés
Glaría Ezquer, Idoia
Andrés, Ximena de
Martínez Pomares, Luisa
Amorena Zabalza, Beatriz
Andrés Cara, Damián de
info:eu-repo/semantics/openAccessAcceso abierto / Sarbide irekia
info:eu-repo/semantics/articleArtículo / Artikulua
info:eu-repo/semantics/publishedVersionVersión publicada / Argitaratu den bertsioa
This study aims to characterize the mannose receptor (MR) gene in sheep and its role in ovine visna/maedi virus (VMV) infection. The deduced amino acid sequence of ovine MR was compatible with a transmembrane protein having a cysteine-rich ricin-type amino-terminal region, a fibronectin type II repeat, eight tandem C-type lectin carbohydrate-recognition domains (CRD), a transmembrane region, and ... [++]
This study aims to characterize the mannose receptor (MR) gene in sheep and its role in ovine visna/maedi virus (VMV) infection. The deduced amino acid sequence of ovine MR was compatible with a transmembrane protein having a cysteine-rich ricin-type amino-terminal region, a fibronectin type II repeat, eight tandem C-type lectin carbohydrate-recognition domains (CRD), a transmembrane region, and a cytoplasmic carboxy-terminal tail. The ovine and bovine MR sequences were closer to each other compared to human or swine MR. Concanavalin A (ConA) inhibited VMV productive infection, which was restored by mannan totally in ovine skin fibroblasts (OSF) and partially in blood monocyte-derived macrophages (BMDM), suggesting the involvement of mannosylated residues of the VMV ENV protein in the process. ConA impaired also syncytium formation in OSF transfected with an ENV-encoding pN3-plasmid. MR transcripts were found in two common SRLV targets, BMDM and synovial membrane (GSM) cells, but not in OSF. Viral infection of BMDM and especially GSM cells was inhibited by mannan, strongly suggesting that in these cells the MR is an important route of infection involving VMV Env mannosylated residues. Thus, at least three patterns of viral entry into SRLV-target cells can be proposed, involving mainly MR in GSM cells (target in SRLV-induced arthritis), MR in addition to an alternative route in BMDM (target in SRLV infections), and an alternative route excluding MR in OSF (target in cell culture). Different routes of SRLV infection may thus coexist related to the involvement of MR differential expression. [--]
Maedi Visna, Nucleotide-sequence, Dendritic cell, Macrophages, Expression, Binding proteins, Carbohydrate, Recognition, Brain
Veterinary Research 2011, 42:28
Universidad Pública de Navarra. Instituto de Agrobiotecnología / Nafarroako Unibertsitate Publikoa. Agrobioteknologiako Institutua
Versión del editor
This work was supported by project CICYT Nos. AGL2007-66874-C04-01/GAN and AGL2010-22341-C04-01. We acknowledge the Public University of Navarra and CSIC for fellowships and contract (HC and RR).
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