Bap, a biofilm matrix protein of Staphylococcus aureus prevents cellular internalization through binding to GP96 host receptor
Fecha
2012Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.1371/journal.ppat.1002843
Resumen
The biofilm matrix, composed of exopolysaccharides, proteins, nucleic acids and lipids, plays a well-known role as a defence
structure, protecting bacteria from the host immune system and antimicrobial therapy. However, little is known about its
responsibility in the interaction of biofilm cells with host tissues. Staphylococcus aureus, a leading cause of biofilmassociated
chronic infections, ...
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The biofilm matrix, composed of exopolysaccharides, proteins, nucleic acids and lipids, plays a well-known role as a defence
structure, protecting bacteria from the host immune system and antimicrobial therapy. However, little is known about its
responsibility in the interaction of biofilm cells with host tissues. Staphylococcus aureus, a leading cause of biofilmassociated
chronic infections, is able to develop a biofilm built on a proteinaceous Bap-mediated matrix. Here, we used the
Bap protein as a model to investigate the role that components of the biofilm matrix play in the interaction of S. aureus with
host cells. The results show that Bap promotes the adhesion but prevents the entry of S. aureus into epithelial cells. A broad
analysis of potential interaction partners for Bap using ligand overlayer immunoblotting, immunoprecipitation with purified
Bap and pull down with intact bacteria, identified a direct binding between Bap and Gp96/GRP94/Hsp90 protein. The
interaction of Bap with Gp96 provokes a significant reduction in the capacity of S. aureus to invade epithelial cells by
interfering with the fibronectin binding protein invasion pathway. Consistent with these results, Bap deficient bacteria
displayed an enhanced capacity to invade mammary gland epithelial cells in a lactating mice mastitis model. Our
observations begin to elucidate the mechanisms by which components of the biofilm matrix can facilitate the colonization
of host tissues and the establishment of persistent infections. [--]
Materias
Staphylococcus aureus,
Bacterial biofilms,
Epithelial cells,
Recombinant proteins,
Mammary glands,
Membrane proteins,
Protein interactions,
Host cells
Editor
Public Library of Science
Publicado en
PLoS Pathogens 8(8): e1002843
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
J.V. and A.T-A were supported by Spanish Ministry of Science and Innovation ‘‘Ramón y Cajal’’ contract. This work was supported by Spanish Ministry of Science and Innovation Grants BIO2008-05284-C02-01, AGL2011-23954 and the grant of Dpto. de Innovación from Gobierno de Navarra IIQ14066.RI1.