Four genes essential for recombination define GInts, a new type of mobile genomic island widespread in bacteria
Bardají Goikoetxea, Leire
Echeverría Ancín, Myriam
Rodríguez Palenzuela, Pablo
Martínez García, Pedro M.
Murillo Martínez, Jesús
Acceso abierto / Sarbide irekiainfo:eu-repo/semantics/openAccess
Artículo / Artikuluainfo:eu-repo/semantics/article
Versión publicada / Argitaratu den bertsioainfo:eu-repo/semantics/publishedVersion
Integrases are a family of tyrosine recombinases that are highly abundant in bacterial genomes, actively disseminating adaptive characters such as pathogenicity determinants and antibiotics resistance. Using comparative genomics and functional assays, we identified a novel type of mobile genetic element, the GInt, in many diverse bacterial groups but not in archaea. Integrated as genomic islands, ... [++]
Integrases are a family of tyrosine recombinases that are highly abundant in bacterial genomes, actively disseminating adaptive characters such as pathogenicity determinants and antibiotics resistance. Using comparative genomics and functional assays, we identified a novel type of mobile genetic element, the GInt, in many diverse bacterial groups but not in archaea. Integrated as genomic islands, GInts show a tripartite structure consisting of the ginABCD operon, a cargo DNA region from 2.5 to at least 70 kb, and a short AT-rich 3′ end. The gin operon is characteristic of GInts and codes for three putative integrases and a small putative helix-loop-helix protein, all of which are essential for integration and excision of the element. Genes in the cargo DNA are acquired mostly from phylogenetically related bacteria and often code for traits that might increase fitness, such as resistance to antimicrobials or virulence. GInts also tend to capture clusters of genes involved in complex processes, such as the biosynthesis of phaseolotoxin by Pseudomonas syringae. GInts integrate site-specifically, generating two flanking direct imperfect repeats, and excise forming circular molecules. The excision process generates sequence variants at the element attachment site, which can increase frequency of integration and drive target specificity. [--]
Mobile genetic element, Phaseolotoxin, Antimetabolite toxin, Integrons, Virulence genes, Horizontal gene transfer
Nature Publishing Group
Scientific Reports 7:46254
Incluye 9 ficheros de datos
Universidad Pública de Navarra. Departamento de Producción Agraria / Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
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This work was funded by the Spanish Plan Nacional I+ D+ i grants AGL2011-30343-C02-02 and AGL2014- 53242-C2-2-R, from the Ministerio de Economía y Competitividad (MINECO), co-financed by the Fondo Europeo de Desarrollo Regional (FEDER).
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