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Rat cytochrome P450-mediated transformation of dichlorodibenzo-Pdioxins by recombinant white-rot basidiomycete Coriolus hirsutus
dc.creator | Shishido, Kazuo | es_ES |
dc.date.accessioned | 2018-02-14T09:31:29Z | |
dc.date.available | 2018-02-14T09:31:29Z | |
dc.date.issued | 2006 | |
dc.identifier.isbn | 84-9769-107-5 | |
dc.identifier.uri | https://hdl.handle.net/2454/27211 | |
dc.description | Comunicación presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005. | es_ES |
dc.description.abstract | Rat cytochrome P450, CYP1A1, has been reported to play an important role in the metabolism of mono-trichlorodibenzo-p-dioxins (M-TriCDDs). To breed lignin (and M-TetraCDDs)-degrading basidiomycete Coriolus hirsutus strains producing rat CYP1A1, an expression cassette [C. hirsutus gpd promoter-C. hirsutus gpd 5’-portion (224-bp of 1st exon-8th base of 4th exon)-rat cyp1a1 cDNALentinula edodes priA terminator] was constructed and inserted into pUCR1 carrying the C. hirsutus arg1 gene. The resulting recombinant plasmid, MIp5-(cyp1a1+arg1) was introduced into protoplasts of C. hirsutus monokaryotic strain OJ1078 (Arg-, Leu-), obtaining three good Arg+ transformants. These transformants [ChTF5-2(CYP1A1), ChTF5-4(CYP1A1), and ChTF5-6(CYP1A1)] were estimated to carry nine, six, and seven copies of the expression cassette on their chromosomes, respectively. Immunoblot analysis revealed that the three transformants produce similar amounts of rat CYP1A1 enzyme. ChTF5-2(CYP1A1), ChTF5-4(CYP1A1), ChTF5-6(CYP1A1) and recipient OJ1078 were cultivated in a liquid medium containing 2,7/2,8 (at a ratio of 1:1)-dichlorodibenzo-pdioxins (2,7/2,8-DCDDs) and the amount of intra- and extracellular 2,7/2,8-DCDDs remaining was measured. The results showed that all three transformants efficiently transform 2,7/2,8-DCDDs through the action of the recombinant rat CYP1A1 enzyme. | en |
dc.description.sponsorship | This work was partly supported by research grants from RITE (Research Institute of Innovative Technology of Earth), NEDO (New Energy and Industrial Technology Development Organization) and Noda Institute for Scientific Research. | en |
dc.format.extent | 10 p. | |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa | es |
dc.relation.ispartof | Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006. | es |
dc.rights | © Autores; Universidad Pública de Navarra. Esta publicación no puede ser reproducida, almacenada o transmitida total o parcialmente, sea cual fuere el medio y el procedimiento, incluidas las fotocopias, sin permiso previo concedido por escrito por los titulares del copyright. | es_ES |
dc.subject | Coriolus hirsutus | en |
dc.subject | Rat cytochrome P450 | en |
dc.title | Rat cytochrome P450-mediated transformation of dichlorodibenzo-Pdioxins by recombinant white-rot basidiomycete Coriolus hirsutus | en |
dc.type | info:eu-repo/semantics/conferenceObject | en |
dc.type | Contribución a congreso / Biltzarrerako ekarpena | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.type.version | Versión publicada / Argitaratu den bertsioa | es |