Sequence analysis and expression of a RecQ gene homologue from Lentinula edodes
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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nodoi-noplumx
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Resumen
We cloned and sequenced a recQ gene homologue from Lentinula edodes. This gene, named Le.recQ, was
found to have a coding capacity of 945 amino acids (aa). The deduced Le.RECQ protein was clearly
smaller than other fungal RecQ proteins such as Neurospora crassa QDE3 (1955 aa), Schizosaccharomyces
pombe Rqh1 (1328 aa), and Saccharomyces cerevisiae SGS1 (1447 aa). It exhibited the highest homolo ...
[++]
We cloned and sequenced a recQ gene homologue from Lentinula edodes. This gene, named Le.recQ, was
found to have a coding capacity of 945 amino acids (aa). The deduced Le.RECQ protein was clearly
smaller than other fungal RecQ proteins such as Neurospora crassa QDE3 (1955 aa), Schizosaccharomyces
pombe Rqh1 (1328 aa), and Saccharomyces cerevisiae SGS1 (1447 aa). It exhibited the highest homology
to the Arabidopsis thaliana RecQl4A protein (1182 aa) in its size and aa sequence. Northern-blot analysis
showed that the Le.recQ gene is transcribed at similar levels during mycelial development in L. edodes
fruiting-body formation. The L. edodes dikaryotic mycelial cells were found to contain a clearly larger
amount of Le.recQ transcript than the L. edodes two compatible monokaryotic mycelial cells. Results
in situ RNA-RNA hybridization showed that subhymenium and outer region of trama contain larger
amounts of Le.recQ transcript. Expression of Le.recQ cDNA in S. cerevisiae might partially complement
defects associated with the loss of its homologue S. cerevisiae SGS1 gene. [--]
Materias
Lentinula edodes,
Le.recQ gene
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Comunicación presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
Entidades Financiadoras
This work was partly supported by research grants from the Ministry of Education,
Culture, Sports, Science, and Technology of Japan.