Heterologous expression of mating type genes in basidiomycetes
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
Mating type genes in basidiomycetes encode two types of transcription factors (HD1 and HD2) and
pheromones and pheromone receptors. Usually, mating type genes are so dissimilar in DNA sequence (allelic
genes and genes from different species) that they do not cross-hybridize. In homobasidiomycetes, directly
next to the A mating type locus encoding the transcription factors is a highly conserved ...
[++]
Mating type genes in basidiomycetes encode two types of transcription factors (HD1 and HD2) and
pheromones and pheromone receptors. Usually, mating type genes are so dissimilar in DNA sequence (allelic
genes and genes from different species) that they do not cross-hybridize. In homobasidiomycetes, directly
next to the A mating type locus encoding the transcription factors is a highly conserved gene mip
that allows positional cloning. A candidate gene for positional cloning of the B mating type genes encoding
the pheromone-pheromone receptor system is cla4/ste20. With more and more mating type loci
cloned from different species, evolution of these loci and their genes can be addressed by sequence analysis
and by function by transformation into other species, here Coprinopsis cinerea. Transformation of
cloned mating type genes into heterologous hosts can lead to activation of mating type controlled development.
Heterologous expression of mating type genes is especially interesting for species in which no
transformation system exists. Since in C. cinerea an A null-mutant is available without functional transcription
factor genes, self-compatibility of cloned A genes from homothallic species can also be tested. [--]
Materias
Basidiomycetes,
Mating type genes
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Comunicación presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
Entidades Financiadoras
PS and WC hold PhD scholarships of the Mahasarakham University (Thailand)
and the Rajamangala University (Thailand), respectively. The Göttingen
laboratory is supported by the Deutsche Bundesstiftung Umwelt
(DBU).