Expression of mating type genes in heterologous basidiomycetes
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
Mating processes in basidiomycetes are controlled by genes encoding two
types of transcription factors (HD1 and HD2) and by genes encoding
pheromones and pheromone receptors. For a successful mating reaction, an
HD1 protein and an HD2 protein of different specificity have to interact
and a pheromone with a pheromone receptor of different specificity. With
now having cloned mating type loci f ...
[++]
Mating processes in basidiomycetes are controlled by genes encoding two
types of transcription factors (HD1 and HD2) and by genes encoding
pheromones and pheromone receptors. For a successful mating reaction, an
HD1 protein and an HD2 protein of different specificity have to interact
and a pheromone with a pheromone receptor of different specificity. With
now having cloned mating type loci from several different basidiomycetes,
evolution of these loci and their genes can be addressed by sequence analysis
as well as by transformation of genes into heterologous species.
Transformation of cloned mating type genes into strains of the same species
with different mating type genes can activate mating type controlled development.
The A mating type genes of Coprinopsis scobicola and of Coprinellus disseminatus
were found to be functional in Coprinopsis cinerea in combination
with the endogenous A mating type genes. Moreover, B genes of C. disseminatus
in C. cinerea cause peg formation subapical to septa with A-induced clamps
cells and fusion of clamp cells with the subapical peg. In several C. cinerea
monokaryons, transformed A genes of Schizophyllum commune were not observed
to induce clamp cells by interaction with endogenous A genes. However,
in crosses of C. cinerea transformants carrying compatible S. commune A
genes, clamp cell production has occasionally been observed. To our surprise,
when using S. commune A genes or the homologous b genes of Ustilago maydis
to transform a specific C. cinerea monokaryon, colonies of transformants may
develop faster growing sectors with hyphae having clamp cells.
Our laboratory is supported by Deutsche Bundesstiftung Umwelt (DBU)
and scholarships by the Mahasarakham University (to PS) and the Rajamangala
Institute of Technology (to WC). [--]
Materias
Heterologous basidiomycetes,
Mating type genes
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen de la conferencia presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
Entidades Financiadoras
The author's laboratory is supported by Deutsche Bundesstiftung Umwelt (DBU). Scholarships by the Mahasarakham University (to PS) and the Rajamangala
Institute of Technology (to WC).