QTL mapping of pathogenicity in Heterobasidion annosum sensu lato
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
Heterobasidion annosum (Fr.) Bref. sensu lato, casual agent of annosum root
rot in conifers, is the economical most devastating forest pathogen in the
northern hemisphere. A genetic linkage map of H. annosum, was constructed
from a compatible mating between isolates from the North American S
and P intersterility groups. The linkage analysis of 358 AFLP markers in 102
progeny isolates generat ...
[++]
Heterobasidion annosum (Fr.) Bref. sensu lato, casual agent of annosum root
rot in conifers, is the economical most devastating forest pathogen in the
northern hemisphere. A genetic linkage map of H. annosum, was constructed
from a compatible mating between isolates from the North American S
and P intersterility groups. The linkage analysis of 358 AFLP markers in 102
progeny isolates generated 19 linkage groups containing 6 or more markers
that covered 1468 cM.
Two distinct methods were used to analyse the segregation of pathogenicity.
1 year old pine seedlings grown in the greenhouse were infected with H.
annosum progeny isolates. Pathogenicity was measured as mean necrosis
lengths caused in 10 pine plants. One QTL for pathogenicity was found on
linkage group 15 with a LOD peak of 2.95, spanning a 31.2 cM large area.
This QTL explained 14.3% of the variation. Another QTL were found in a
small linkage group containing 5 markers and spanning 36.8 cM, with a
LOD peak of 4.40 at marker paacts02. 19.1% of the variation could be explained
by this QTL. The heritability of pathogenicity on pine was estimated
to be 0.21 in this study.
The disease increase rate values from an in vitro test were used as another
estimate of pathogenicity and used for the QTL-analysis. From the in vitro
pathogenicity test, a large area of 26.5 cM on linkage group 11 between the
markers acgcs5 and paacgp08 contains a high QTL probability of LOD 3.09.
This QTL explained 16.4% of the total variation in this experiment. The
heritability of pathogenicity on pine was estimated to be 0.088 in this study.
Successfully localization of the two intersterility genes (S and P) on the
map were carried out through mating of the progeny isolates with three
tester strains carrying known intersterility genotypes. [--]
Materias
Heterobasidion annosum,
QTL,
Pathogenicity
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen de la conferencia presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.