Expression of GFP and DsRed in the homobasidiomycete Schizophyllum commune
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
Expression of GFP and DsRed was studied in the homobasidiomycete
Schizophyllum commune. CGFP or sGFP fused to the SC3 promoter resulted
in similar steady state mRNA levels. These levels were considerably lower
than that of SC3. Despite the low mRNA levels, both GFP variants resulted
in fluorescent hyphae. The sGFP expressing strains showed stronger fluorescence
than the CGFP expressers. When ...
[++]
Expression of GFP and DsRed was studied in the homobasidiomycete
Schizophyllum commune. CGFP or sGFP fused to the SC3 promoter resulted
in similar steady state mRNA levels. These levels were considerably lower
than that of SC3. Despite the low mRNA levels, both GFP variants resulted
in fluorescent hyphae. The sGFP expressing strains showed stronger fluorescence
than the CGFP expressers. When CGFP was fused to the N-terminal
or C-terminal part of the mature SC3 protein, no fluorescence was observed.
However, accumulation of GFP [--]
Materias
GFP,
DsRed,
Schizophyllum commune
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen de la conferencia presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.