The roles of SC3 and SC15 of Schizophyllum commune during growth on wood
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
The SC3 hydrophobin plays several roles in growth and development of S.
commune. It lowers the surface tension of the aqueous substrate, enabling aerial
growth, and it coats the aerial hyphae rendering them hydrophobic. SC3
also allows hyphae to attach to hydrophobic surfaces. Moreover, SC3 has a
role in the cell wall architecture. In the absence of the hydrophobin, S. commune
produces more ...
[++]
The SC3 hydrophobin plays several roles in growth and development of S.
commune. It lowers the surface tension of the aqueous substrate, enabling aerial
growth, and it coats the aerial hyphae rendering them hydrophobic. SC3
also allows hyphae to attach to hydrophobic surfaces. Moreover, SC3 has a
role in the cell wall architecture. In the absence of the hydrophobin, S. commune
produces more mucilage. The SC15 protein mediates aerial hyphae
formation and attachment in the absence of SC3. Besides being secreted into
the medium, the protein can be found in the mucilage that binds aerial hyphae
together. So far, studies were performed on minimal media. We here assessed
the roles of SC3 and SC15 during growth on wood. SC3 and SC15
were shown not to play a role in colonization of wood. Biomass formation
and radial extension was similar in the wild-type and in strains in which either
or both SC3 and SC15 were deleted. Interestingly, in contrast to growth
on minimal medium deletion of SC15 alone affected formation of aerial hyphae.
A similar reduction was observed for the SC3 mutant while reduction
in the ∅SC3∅SC15 was most dramatic. At the moment we assess the role of
SC15 in the fruiting body. Preliminary data using GFP as a reporter indicate
that SC15 is expressed within the fruiting body. Here it may play a role in the
mucilage surrounding the hyphae. [--]
Materias
SC3,
SC15,
Schizophyllum commune
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen de la conferencia presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.