Mitogenic activated protein kinase Kpp6 signaling in the phytopathogenic Fungus Ustilago maydis: identification of downstream elements
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
Pathogenicity of U.maydis is dependent on its ability to mate. Mating requires
active cAMP-PKA and MAP kinase cascades and except for the signaling
inputs the downstream components are also required during pathogenesis.
In addition a MAP kinase, called Kpp6 was described, that works in
pathogenicty but not in mating. Kpp6 displays high similarity to Kpp2, the
MAP kinase that works in mating. ...
[++]
Pathogenicity of U.maydis is dependent on its ability to mate. Mating requires
active cAMP-PKA and MAP kinase cascades and except for the signaling
inputs the downstream components are also required during pathogenesis.
In addition a MAP kinase, called Kpp6 was described, that works in
pathogenicty but not in mating. Kpp6 displays high similarity to Kpp2, the
MAP kinase that works in mating. kpp6 mutants were morphologically indistinguishable
from wild type but were unable to induce anthocyanin production
and were unable to penetrate into the plant, despite the fact that they
did produce appressoria. In this work we are studying the downstream elements
of Kpp6 and determine their function during the penetration process.
To obtain this information we used microarray assays with RNA from Ustilago
growing on plant surface. We identified 29 genes which are reduced in
expression in Kpp6 mutant with respect the wild type strain. We generated
knockout strains for some of these genes in compatible strains of U. maydis
and analyzed the phenotypes in pathogenicity. Our preliminary results suggest
that Kpp6 regulates the penetration of Ustilago maydis at the stage where
lytic enzymes expression is no longer required. [--]
Materias
MAP kinase Kpp6,
Ustilago maydis
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen del poster presentado al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.