Show simple item record

dc.creatorBorgognone, Alessandraes_ES
dc.creatorCastanera Andrés, Raúles_ES
dc.creatorMuguerza Domínguez, Elaiaes_ES
dc.creatorPisabarro de Lucas, Gerardoes_ES
dc.creatorRamírez Nasto, Lucíaes_ES
dc.date.accessioned2018-09-04T06:43:43Z
dc.date.available2018-09-04T06:43:43Z
dc.date.issued2017
dc.identifier.issn1340-2838,1756-1663
dc.identifier.urihttps://hdl.handle.net/2454/30421
dc.description.abstractHelitrons constitute a superfamily of DNA transposons that were discovered in silico and are widespread in most eukaryotic genomes. They are postulated to mobilize through a “rollingcircle” mechanism, but the experimental evidence of their transposition has been described only recently. Here, we present the inheritance patterns of HELPO1 and HELPO2 helitron families in meiotically derived progeny of the basidiomycete Pleurotus ostreatus. We found distorted segregation patterns of HELPO2 helitrons that led to a strong under-representation of these elements in the progeny. Further investigation of HELPO2 flanking sites showed that gene conversion may contribute to the elimination of such repetitive elements in meiosis, favouring the presence of HELPO2 vacant loci. In addition, the analysis of HELPO2 content in a reconstructed pedigree of subclones maintained under different culture conditions revealed an event of helitron somatic transposition. Additional analyses of genome and transcriptome data indicated that P. ostreatus carries active RNAi machinery that could be involved in the control of transposable element proliferation. Our results provide the first evidence of helitron mobilization in the fungal kingdom and highlight the interaction between genome defence mechanisms and invasive DNA.en
dc.description.sponsorshipThis work was supported by Spanish National Research Plan (Projects AGL2011-30495 and AGL2014-55971-R) and FEDER funds; Public University of Navarre (http://www.unavarra.es); AB holds a PhD scholarship from the Public University of Navarre and RC holds a FPI-PhD scholarship from the Ministry of Economy and Competitiveness.en
dc.format.extent13 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherOxford University Pressen
dc.relation.ispartofDNA Research, 2017, 24(2), 103–115en
dc.rights© The Author(s) 2017. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comen
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectHelitronsen
dc.subjectSegregation distortionen
dc.subjectGene conversionen
dc.subjectTransposable elementsen
dc.subjectBasidiomyceteen
dc.titleSomatic transposition and meiotically driven elimination of an active helitron family in Pleurotus ostreatusen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1093/dnares/dsw060
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/AGL2011-30495en
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/1PE/AGL2014–55971-Ren
dc.relation.publisherversionhttps://doi.org/10.1093/dnares/dsw060
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

© The Author(s) 2017. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
Except where otherwise noted, this item's license is described as © The Author(s) 2017. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com