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dc.creatorBeperet Arive, Inéses_ES
dc.creatorBarrera Cubillos, Gloria Patriciaes_ES
dc.creatorSimón de Goñi, Oihanees_ES
dc.creatorWilliams, Trevores_ES
dc.creatorLópez Ferber, Migueles_ES
dc.creatorGasmi, Lailaes_ES
dc.creatorHerrero, Salvadores_ES
dc.creatorCaballero Murillo, Primitivoes_ES
dc.date.accessioned2018-09-06T12:20:19Z
dc.date.available2018-09-06T12:20:19Z
dc.date.issued2013
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/2454/30547
dc.description.abstractA recombinant virus lacking the sf32 gene (Sf32null), unique to the Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), was generated by homologous recombination from a bacmid comprising the complete viral genome (Sfbac). Transcriptional analysis revealed that sf32 is an early gene. Occlusion bodies (OBs) of Sf32null contained 62% more genomic DNA than viruses containing the sf32 gene, Sfbac and Sf32null-repair, although Sf32null DNA was three-fold less infective when injected in vivo. Sf32null OBs were 18% larger in diameter and contained 17% more nucleocapsids within ODVs than those of Sfbac. No significant differences were detected in OB pathogenicity (50% lethal concentration), speed-of-kill or budded virus production in vivo. In contrast, the production of OBs/larva was reduced by 39% in insects infected by Sf32null compared to those infected by Sfbac. The SF32 predicted protein sequence showed homology (25% identity, 44% similarity) to two adhesion proteins from Streptococcus pyogenes and a single N-mirystoylation site was predicted. We conclude that SF32 is a non-essential protein that could be involved in nucleocapsid organization during ODV assembly and occlusion, resulting in increased numbers of nucleocapsids within ODVs.es
dc.description.sponsorshipThis study received financial support from the Spanish Ministry for Science and Technology (AGL2008-05456-C03-01, AGL2011-30352-C02-01). I.B. received a CSIC studentship.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.relation.ispartofPlos One, 8(10): e77683en
dc.rights© 2013 Beperet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.subjectSpodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV)es
dc.subjectsf32es
dc.subjectNucleocapsid organizationes
dc.subjectOcclusion-derived virionses
dc.titleThe sf32 unique gene of spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) is a non-essential gene that could be involved in nucleocapsid organization in occlusion-derived virionsen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.contributor.departmentIdAB – Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1371/journal.pone.0077683
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/AGL2011-30352en
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0077683
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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© 2013 Beperet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as © 2013 Beperet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.