Co-infection with iflaviruses influences the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus occlusion bodies: implications for the production and biosecurity of baculovirus insecticides
Fecha
2017Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1371/journal.pone.0177301
Resumen
Biological insecticides based on Spodoptera exigua multiple nucleopolyhedrovirus
(SeMNPV) can efficiently control S. exigua larvae on field and greenhouse crops in many
parts of the world. Spanish wild populations and laboratory colonies of S. exigua are infected
by two iflaviruses (SeIV-1 and SeIV-2). Here we evaluated the effect of iflavirus co-infection
on the insecticidal characteristics ...
[++]
Biological insecticides based on Spodoptera exigua multiple nucleopolyhedrovirus
(SeMNPV) can efficiently control S. exigua larvae on field and greenhouse crops in many
parts of the world. Spanish wild populations and laboratory colonies of S. exigua are infected
by two iflaviruses (SeIV-1 and SeIV-2). Here we evaluated the effect of iflavirus co-infection
on the insecticidal characteristics of SeMNPV occlusion bodies (OBs). Overall, iflavirus coinoculation
consistently reduced median lethal concentrations (LC50) for SeMNPV OBs
compared to larvae infected with SeMNPV alone. However, the speed of kill of SeMNPV
was similar in the presence or absence of the iflaviruses. A reduction of the weight gain
(27%) associated with iflavirus infection resulted in a 30% reduction in total OB production
per larva. Adult survivors of SeMNPV OB inoculation were examined for covert infection.
SeMNPV DNA was found to be present at a high prevalence in all SeIV-1 and SeIV-2 coinfection
treatments. Interestingly, co-inoculation of SeMNPV with SeIV-2 alone or in mixtures
with SeIV-1 resulted in a significant increase in the SeMNPV load of sublethally
infected adults, suggesting a role for SeIV-2 in vertical transmission or reactivation of sublethal
SeMNPV infections. In conclusion, iflaviruses are not desirable in insect colonies used
for large scale baculovirus production, as they may result in diminished larval growth,
reduced OB production and, depending on their host-range, potential risks to non-target
Lepidoptera. [--]
Materias
SeMNPV occlusion bodies,
Iflavirus co-infection,
Biological insecticides
Editor
Public Library of Science
Publicado en
Plos One, 12(5):e0177301
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This study was funded by the Spanish Ministerio de Ciencia e Innovación, project number AGL2011-30352-C02-01. AC received a student scholarship from the Ministerio de Economía y Competitividad.