Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment
Fecha
2013Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1371/journal.pone.0062771
Resumen
To determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9-cis RA
or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers induced apoptosis and reduced
cell density as a result of cell differentiation and/or apoptosis. Flow cytometry revealed that 9-cis RA induced apoptosis
more effectively than AT ...
[++]
To determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9-cis RA
or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers induced apoptosis and reduced
cell density as a result of cell differentiation and/or apoptosis. Flow cytometry revealed that 9-cis RA induced apoptosis
more effectively than ATRA. The expression profile of apoptosis and survival pathways was cell line specific and depended
on the isomer used. [--]
Materias
Retinoic acid,
Neuroblastoma,
Apoptosis
Editor
Public Library of Science
Publicado en
Plos One, 8(5): e62771
Departamento
Universidad Pública de Navarra. Departamento de Ciencias de la Salud /
Nafarroako Unibertsitate Publikoa. Osasun Zientziak Saila
Versión del editor
Entidades Financiadoras
This work was supported by grants from the Departmento de Salud, Gobierno de Navarra, Pamplona, and from the Fondo de Investigación Sanitaria
(PI08/1849), Madrid, Spain.