Relative contribution of P5 and hap surface proteins to nontypable haemophilus influenzae interplay with the host upper and lower airways
Fecha
2015Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.1371/journal.pone.0123154
Resumen
Nontypable Haemophilus influenzae (NTHi) is a major cause of opportunistic respiratory
tract disease, and initiates infection by colonizing the nasopharynx. Bacterial surface proteins
play determining roles in the NTHi-airways interplay, but their specific and relative contribution
to colonization and infection of the respiratory tract has not been addressed
comprehensively. In this study, we ...
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Nontypable Haemophilus influenzae (NTHi) is a major cause of opportunistic respiratory
tract disease, and initiates infection by colonizing the nasopharynx. Bacterial surface proteins
play determining roles in the NTHi-airways interplay, but their specific and relative contribution
to colonization and infection of the respiratory tract has not been addressed
comprehensively. In this study, we focused on the ompP5 and hap genes, present in all H.
influenzae genome sequenced isolates, and encoding the P5 and Hap surface proteins, respectively.
We employed isogenic single and double mutants of the ompP5 and hap genes
generated in the pathogenic strain NTHi375 to evaluate P5 and Hap contribution to biofilm
growth under continuous flow, to NTHi adhesion, and invasion/phagocytosis on nasal, pharyngeal,
bronchial, alveolar cultured epithelial cells and alveolar macrophages, and to NTHi
murine pulmonary infection.We show that P5 is not required for bacterial biofilm growth, but
it is involved in NTHi interplay with respiratory cells and in mouse lung infection. Mechanistically,
P5NTHi375 is not a ligand for CEACAM1 or α5 integrin receptors. Hap involvement in
NTHi375-host interaction was shown to be limited, despite promoting bacterial cell adhesion
when expressed in H. influenzae RdKW20.We also show that Hap does not contribute
to bacterial biofilm growth, and that its absence partially restores the deficiency in lung infection
observed for the ΔompP5 mutant. Altogether, this work frames the relative importance
of the P5 and Hap surface proteins in NTHi virulence. [--]
Materias
Nontypable Haemophilus influenzae (NTHi),
ompP5 genes,
Hap genes
Editor
Public Library of Science
Publicado en
Plos One, 10(4): e0123154
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This study was supported by ISCIII PS09/00130, Ministerio Economía y Competitividad-MINECO SAF2012-31166, Dpto. Salud Gobierno Navarra 359/2012, CIBERES.