Adiposity and adipogenic gene expression in four different muscles in beef cattle
Date
2017Author
Version
Acceso abierto / Sarbide irekia
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Artículo / Artikulua
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Versión publicada / Argitaratu den bertsioa
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10.1371/journal.pone.0179604
Abstract
Anatomical site and divergent functionalities of muscles can be related to differences in IMF
content, metabolism and adipogenic gene expression. Then, potential differences in different
muscles in beef cattle were studied. As a second objective, the main sources of experimental
variability associated to RT-qPCR results were analyzed following a nested design
in order to implement appropriate ...
[++]
Anatomical site and divergent functionalities of muscles can be related to differences in IMF
content, metabolism and adipogenic gene expression. Then, potential differences in different
muscles in beef cattle were studied. As a second objective, the main sources of experimental
variability associated to RT-qPCR results were analyzed following a nested design
in order to implement appropriate experimental designs minimizing gene expression variability.
To perform the study Longissimus thoracis (LT), Semitendinosus (SM), Masseter
(MS), Sternomandibularis (ST) and subcutaneous adipose tissue (SAT) samples of Pirenaica
young bulls (n = 4) were collected for IMF, collagen and protein quantification,
analysis of adipocyte size distribution and gene expression (PPARG, CEBPA, FAPB4 and
WNT10B). A greater IMF content was observed in MS and SM muscles, which had a
bimodal adipocyte size distribution while it was unimodal in the muscles LT and ST. This
suggest that the different IMF accretion in the muscles studied might be related to different
rates of hyperplasia and hypertrophy and that IMF might develop later in LT and ST muscles.
The former differences were not mirrored by the expression of the genes analyzed,
which might be related to the different contribution of mature and non-mature adipocytes to
the total gene expression. When comparing IMF and SAT gene expression, late and early
developing tissues respectively, expression of PPARG, CEBPA and FABP4 was higher in
the SAT, in agreement with bigger cell size and numbers. The variability study indicates that
the analytical factors that add higher variability to the gene expression are the sampling and
RT and therefore, it would be appropriate to include those replicates in the design of future
experiments. Based on the results, the use of MS and SM muscles could allow less expensive
experimental designs and bigger sample size that could permit the detection of lower
relevant differences in gene expression. [--]
Publisher
Public Library of Science
Published in
Plos One, 12(6): e0179604
Departament
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
Publisher version
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